Current bioaccumulation regulation is focused on bioconcentration in fish. An extension to terrestrial mammals, e.g. rat, is urgently needed but will have to use a different metric, most likely the BMF. While both metrics are thermodynamically not equivalent the regulative testing requirements for both might be reduced to the investigation of the respective elimination rate constants k₂ for fish or rat. These k₂ values could be derived from animal tests or from in vitro - in vivo extrapolation and could be combined with estimated uptake rate constants to yield either a BCF or a BMF value. The possibility to use in vitro methods for k₂ has the advantage that animal tests can be avoided and it bears the chance to experimentally cover species differences which are currently ignored in bioaccumulation regulation. Existing data for BCF and the respective k₂ values for fish - either from feeding studies or from BCF studies themselves-indicate that this approach works. For terrestrial bioaccumulation this approach still needs further experimental support.

当前的生物蓄积法规侧重于鱼类中的生物富集。迫切需要扩展到陆地哺乳动物（例如大鼠），但是必须使用其他度量标准，最有可能是BMF。虽然这两个指标在热力学上不相等，但对于这两个指标的调节测试要求可能会降低到研究鱼类或大鼠各自消除速率常数k ₂的水平。这些k ₂值可以源自动物试验或体外-体内外推法，并且可以与估计的摄取速率常数结合以产生BCF或BMF值。对k ₂使用体外方法的可能性这样做的优点是可以避免动物试验，并且有机会通过实验覆盖目前在生物蓄积调节中忽略的物种差异。BCF的现有数据以及鱼类的相应k ₂值-来自饲喂研究或来自BCF研究本身，均表明该方法有效。对于陆地生物富集，这种方法仍需要进一步的实验支持。