Increasing metal consumption is driving the introduction of new techniques such as biomining to exploit low grade ores. The biomining impacts notably aquatic ecosystems, yet, the applicability of ecotoxicological tests to study the complex mixture effects of mining waters is insufficiently understood. The aim of the present work was to test if transcriptomic biomarkers are suitable and sensitive for the ecotoxicity assessment of biomining affected waters. The study site had been affected by a multimetal biomine, and the studied water samples formed a concentration gradient of contamination downstream from the biomining site. Cadmium and nickel were used as positive controls in the toxicity tests. Selected transcriptomic biomarkers, previously shown to be differentially regulated by metals, were used to evaluate the ecotoxicity of the water samples. Parallel samples were used to compare the transcriptomic biomarkers with the conventional acute D. magna toxicity test. In the acute test, one sample was acutely toxic to D. magna, when pH was adjusted according to the standard, whereas, in the native pH, three samples caused total immobility. Monooxygenase was up-regulated by the highest concentration of Cd in control samples and three of the water samples. Vtg-SOD was up-regulated by one of the water samples, and catalase by the second highest concentration of Cd. The results show that transcriptomic biomarkers in D. magna can be used as sensitive bioindicators for metal mixture toxicity assessment in complex environmental water samples.

金属消耗量的增加正推动着新技术的引入，例如利用生物开采来开采低品位矿石。生物采矿对水生生态系统的影响尤其显着，然而，对于研究矿井水的复杂混合效应的生态毒理学测试的适用性却知之甚少。本工作的目的是测试转录组生物标志物是否适合并敏感地用于生物开采受影响水域的生态毒性评估。研究地点受到了多金属生物矿的影响，所研究的水样本在生物开采地点的下游形成了污染物的浓度梯度。镉和镍在毒性试验中用作阳性对照。先前显示出受金属差异调节的选定转录组生物标志物，用于评估水样的生态毒性。D. magna毒性试验。在急性试验中，按照标准调节pH值时，一个样品对D. magna急性毒性，而在天然pH中，三个样品导致完全不动。对照样品和三个水样品中镉的最高浓度上调了单加氧酶。Vtg-SOD被其中一种水样品上调，过氧化氢酶被第二高浓度的Cd上调。结果表明，在转录生物标记物D.蚤可以用作用于复杂环境水样中的金属混合物毒性评估敏感生物指标。