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Spectrophotometric and Digital Colour Colourimetric (DCC) analysis of colour-based indicators
Sensors and Actuators B: Chemical ( IF 8.0 ) Pub Date : 2018-07-05 , DOI: 10.1016/j.snb.2018.06.131
Dilidaer Yusufu , Andrew Mills

Seven simulated absorption spectra that span the visible spectrum, are used to probe the degree of linear correlation that exists between real absorbance, Ao, at λmax, and three well-established colour-based parameters, based on the standard Red, Green and Blue scale, sRGB, namely: (i) apparent absorbance, A(sRGB), (ii) apparent fraction of absorbed light, 1-T(sRGB), where T is the apparent transmittance and (iii) colour difference, ΔE. In all cases the colour-based parameter, A(sRGB), linearly correlates best with Ao. This predicted correlation is tested using three different, actual colour-based indicators, using UV/Vis absorption spectroscopy to monitor the change in actual absorbance of each of the indicators and digital photography to monitor simultaneously the change in the values of sRGB, and so A(sRGB). The three different indicators used were: a CO2 indicator, a photocatalytic activity indicator and an oxygen indicator. In all three cases the apparent absorbance parameter, A(sR), derived from sRGB analysis of the digital images, is proportional to the real absorbance, as measured using UV/Vis spectrophotometry, and able to yield the same key analytical information. The increasing use of sRGB analysis of digital photographic images, i.e. digital colour colourimetry, DCC, is discussed briefly.



中文翻译:

分光光度和数字色度(DCC)分析基于颜色的指示剂

七个跨越可见光谱的模拟吸收光谱,用于探测真正的吸光度,之间存在线性相关程度Ó,在λ最大和三个公认基于颜色的参数,基于标准的红,绿和蓝标sRGB,即:(i)表观吸收率A(sRGB),(ii)吸收光的表观分数1-T(sRGB),其中T是表观透射率,(iii)色差ΔE。在所有情况下,基于颜色的参数A(sRGB)与A o线性最佳相关。使用三种不同的基于实际颜色的指示剂,通过UV / Vis吸收光谱法监测每个指示剂的实际吸光度变化以及使用数字摄影技术同时监测sRGB值的变化来测试此预测的相关性,因此A (sRGB)。使用的三种不同的指示剂是:CO 2指示剂,光催化活性指示剂和氧气指示剂。在所有三种情况下,从数字图像的sRGB分析得出的表观吸光度参数A(sR)与使用UV / Vis分光光度法测得的真实吸光度成比例,并且能够产生相同的关键分析信息。sRGB的使用越来越多 简要讨论了数字摄影图像的分析,即数字彩色比色法DCC。

更新日期:2018-07-05
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