Carbon quantum dots (CQDs) were prepared from wood soot, and the CQDs could catalyze the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) in the presence of H₂O₂ to form oxidized TMB (ox-TMB) with an absorption peak at 652 nm. It was found that the introduction of glutathione (GSH) could cause the reduction of ox-TMB, resulting in a decrease of the absorbance at 652 nm. Based on this, a highly sensitive colorimetric sensor for detection of GSH was fabricated. The influences of various experimental variables, including pH, temperature, and the concentrations of H₂O₂, CQDs and TMB, on the analytical performance of the method were systematically investigated. Under the conditions of pH 3.5, temperature 35 °C, H₂O₂ 1.0 mM, CQDs 2.5 μg/mL and TMB 0.5 mM, the response was linearly proportional to the concentration of GSH within the range of 0.05 to 20 μM with a low detect limit (3 σ/k) of 0.016 μM. The method was successfully applied to determination of GSH in human serum samples with the recovery of 95.7%–103.6%.

由木烟灰制备碳量子点（CQDs），该碳量子点可在H ₂ O ₂存在下催化3，3'，5，5'-四甲基联苯胺（TMB）的氧化，形成氧化的TMB（ox-TMB ）在652 nm处有一个吸收峰。发现谷胱甘肽（GSH）的引入可能导致ox-TMB的减少，从而导致652 nm处的吸光度降低。基于此，制造了用于检测GSH的高灵敏度比色传感器。系统地研究了pH，温度，H ₂ O ₂，CQDs和TMB浓度等各种实验变量对方法分析性能的影响。在pH 3.5，温度35°C，H _{2的条件下}O ₂ 1.0 mM，CQDs 2.5μg/ mL和TMB 0.5 mM，响应与GSH浓度在0.05至20μM范围内呈线性比例关系，检测限低（3σ/ k）为0.016μM。该方法已成功用于人体血清样品中谷胱甘肽的测定，回收率为95.7％–103.6％。