A novel tetraphenylethene-diketopyrrolopyrrole Schiff base (TPE-DPP) was synthesized by one-pot protocol. Based on thiol group and acid-base property of glutathione, nanoaggregates of TPE-DPP for sensitive and selective detection of diff ;erent concentration ranges of glutathione by two distinct reaction mechanisms was reported. It showed a turn-on mode of fluorescence signals towards low concentration of GSH by hydrolysis of TPE-DPP, but a ratiometric mode of fluorescence signal was elicited towards high concentration of GSH by addition reaction of thiol with TPE-DPP. What’s more, the probe was successfully utilized for monitoring GSH in living cells.

通过一锅法合成了新型的四苯基乙烯-二酮吡咯并吡咯席夫碱（TPE-DPP）。根据谷胱甘肽的巯基和酸碱性质，报道了TPE-DPP纳米聚集体通过两种不同的反应机理灵敏和选择性地检测谷胱甘肽的不同浓度范围。通过TPE-DPP的水解显示出荧光信号向低浓度的GSH的开启模式，但是通过硫醇与TPE-DPP的加成反应，荧光信号向高浓度的GSH呈比例模式。此外，该探针已成功用于监测活细胞中的GSH。