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Bioprocess development for biolubricant production using microbial oil derived via fermentation from confectionery industry wastes
Bioresource Technology ( IF 9.7 ) Pub Date : 2018-07-06 , DOI: 10.1016/j.biortech.2018.07.016
Aikaterini Papadaki , Keysson Vieira Fernandes , Afroditi Chatzifragkou , Erika Cristina Gonçalves Aguieiras , José André Cavalcanti da Silva , Roberto Fernandez-Lafuente , Seraphim Papanikolaou , Apostolis Koutinas , Denise Maria Guimarães Freire

Microbial oil produced from confectionery and wheat milling side streams has been evaluated as novel feedstock for biolubricant production. Nutrient-rich fermentation media were produced by a two-step bioprocess involving crude enzyme production by solid state fermentation followed by enzymatic hydrolysis of confectionery industry waste. Among 5 yeast strains and 2 fungal strains cultivated on the crude hydrolysate, Rhodosporidium toruloides and Cryptococcus curvatus were selected for further evaluation for biolubricant production based on fermentation efficiency and fatty acid composition. The extracted microbial oils were enzymatically hydrolysed and the free fatty acids were esterified by Lipomod 34-MDP in a solvent-free system with trimethylolpropane (TMP) and neopentyl glycol (NPG). The highest conversion yields were 88% and 82.7% for NPG esters of R. toruloides and C. curvatus, respectively. This study also demonstrates that NPG esters produced from microbial oil have promising physicochemical properties for bio-based lubricant formulations that could substitute for conventional lubricants.



中文翻译:

利用糖业发酵废料发酵产生的微生物油生产生物润滑剂的生物工艺开发

从糖果和小麦碾磨侧流产生的微生物油已被评估为生产生物润滑剂的新型原料。通过两步生物过程来生产富含营养的发酵培养基,该过程包括通过固态发酵生产粗酶,然后对糖果工业废料进行酶促水解。在粗水解产物中培养的5种酵母菌株和2种真菌菌株中,球形假单胞菌弯曲隐球菌选择基于发酵效率和脂肪酸组成的用于生物润滑剂生产的进一步评估。将提取的微生物油进行酶水解,并用Lipomod 34-MDP在无溶剂系统中用三羟甲基丙烷(TMP)和新戊二醇(NPG)酯化游离脂肪酸。R. toruloidesC. curvatus的NPG酯的最高转化率分别为88%和82.7%。这项研究还表明,由微生物油生产的NPG酯具有可替代传统润滑剂的生物基润滑剂配方的有前途的理化性质。

更新日期:2018-07-06
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