High-fat diet (HFD) is associated with impairment of insulin sensitivity. We investigated the effects of Bangle (Zingiber purpureum) extract (Ba) with exercise training (Ex) on phosphorylation of Akt and mammalian target of rapamycin (mTOR), and autophagy flux in the gastrocnemius (GM) of HFD-fed rats. The rats, on a diet providing 45% of energy from fat, were divided into four groups: untreated (HFD), 1.5% Ba-treated (HFD + Ba), 1.5% Ba with Ex-treated (HFD + Ba + Ex), and Ex-treated (HFD + Ex) groups. The controls were fed a normal diet. The homeostatic model assessment of insulin resistance index and non-esterified fatty acid levels were decreased in the HFD + Ba + Ex. The HFD + Ba + Ex showed decreased phosphorylated Akt and mTOR levels, and increased levels of autophagy flux markers, LC3B-II and Beclin 1, compared to the HFD. In conclusion, the combination of Ba and Ex modulates Akt/mTOR phosphorylation and the autophagy flux in the GM and may attenuate insulin resistance induced by a HFD.

高脂饮食（HFD）与胰岛素敏感性受损有关。我们调查了手镯（姜姜紫）的影响）通过运动训练（Ex）对Akt的磷酸化和雷帕霉素（mTOR）的哺乳动物靶标进行提取（Ba），并在喂食HFD的大鼠腓肠肌（GM）中自噬通量。以脂肪提供45％能量的饮食为基础，将大鼠分为四组：未处理（HFD），1.5％钡处理（HFD + Ba），1.5％含Ex处理的（HFD + Ba + Ex） ，以及经过治疗的（HFD + Ex）组。对照组接受正常饮食。在HFD + Ba + Ex中，胰岛素抵抗指数和非酯化脂肪酸水平的稳态模型评估降低。与HFD相比，HFD + Ba + Ex显示磷酸化的Akt和mTOR降低，自噬通量标记物LC3B-II和Beclin 1升高。综上所述，