Alcalase was screened out for hydrolyzing the swim bladder proteins of miiuy croaker (Miichthys miiuy) from five proteases using hydroxyl radical scavenging assay, and its optimal hydrolysis conditions were be determined as time 3.5 h, temperature 55 °C, pH 9.5, solid-liquid ratio 1:5, and enzyme dose 2.5%. Ten new antioxidant peptides were isolated from SMAH using ultrafiltration and consecutive chromatography, and their sequences were identified as FYKWP, FTGMD, GFEPY, YLPYA, FPPYERRQ, GFYAA, FSGLR, FPYLRH, VPDDD, and GIEWA. FPYLRH and GIEWA exhibited strong scavenging activities on hydroxyl radical (EC₅₀ 0.68 mg/mL and 0.71 mg/mL for FPYLRH and GIEWA, respectively), DPPH radical (EC₅₀ 0.51 mg/mL and 0.78 mg/mL for FPYLRH and GIEWA, respectively), and superoxide anion radical (EC₅₀ 0.34 mg/mL and 0.30 mg/mL for FPYLRH and GIEWA, respectively). In addition, FPYLRH and GIEWA could be effectively against lipid peroxidation in the β-carotene linoleic acid and in the linoleic acid emulsion system.

使用羟自由基清除法从5种蛋白酶中筛选出Alcalase来水解miiuy croaker（Miichthys miiuy）的游泳膀胱蛋白，并确定最佳水解条件为时间3.5 h，温度55°C，pH 9.5，固液比例为1：5，酶剂量为2.5％。使用超滤和连续色谱法从SMAH中分离出十种新的抗氧化剂肽，其序列分别为FYKWP，FTMGD，GFEPY，YLPYA，FPPYERRQ，GFYAA，FSGLR，FPYLRH，VPDDD和GIEWA。FPYLRH和GIEWA对羟基自由基（FPYLRH和GIEWA的EC _50分别为0.68 mg / mL和0.71 mg / mL），DPPH自由基（EC ₅₀）具有很强的清除活性FPYLRH和GIEWA分别为0.51 mg / mL和0.78 mg / mL）和超氧阴离子自由基（FPYLRH和GIEWA分别为EC ₅₀ 0.34 mg / mL和0.30 mg / mL）。此外，FPYLRH和GIEWA可以有效抵抗β-胡萝卜素亚油酸和亚油酸乳液体系中的脂质过氧化。