Currently, algal fuel research has commenced to shift toward genetically engineered mutants able to express and excrete desired products directly into the culture. In this study, a mutant strain of Chlamydomonas reinhardtii, engineered for bisabolene (alternative biodiesel) excretion, was cultivated at different illumination and temperatures to investigate their effects on cell growth and bisabolene production. Moreover, a kinetic model was constructed to identify the desirable conditions for biofuel synthesis. Three original contributions were concluded. First, this work confirmed that bisabolene was partially synthesized independently of biomass growth, indicating its feasibility for continuous production. Second, it was found that while bisabolene synthesis was independent of light intensity, it was strongly affected by temperature, resulting in conflicting desirable conditions for cell growth and product synthesis. Finally, through model prediction, optimal operating conditions were identified for mutant growth and bisabolene synthesis. This study therefore paves the way toward chemostat production and process scale-up.

中文翻译：

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绿色微藻突变体光催化制备比沙泊烯的过程分析和动力学建模

目前，藻类燃料的研究已开始转向能够将所需产物直接表达和排泄到培养物中的基因工程突变体。在这项研究中，莱茵衣藻的突变株在不同的光照和温度下培养了专为Bisabolene（替代生物柴油）排泄而设计的，以研究它们对细胞生长和Bisabolene产生的影响。此外，建立了动力学模型以识别生物燃料合成的理想条件。总结了三份原始文稿。首先，这项工作证实了比沙泊烯是部分独立于生物质生长而合成的，表明其可连续生产。其次，已发现虽然双油松烯的合成不依赖于光强度，但是它受温度的强烈影响，导致细胞生长和产物合成的理想条件相互矛盾。最后，通过模型预测，确定了突变体生长和双倍半萜合成的最佳操作条件。