An (η⁵-cyclopentadienyl)cobalt(I) complex was covalently incorporated into an engineered variant of the transmembrane protein ferric hydroxamate uptake protein component: A, FhuA ΔCVF^tev, using a thiol–ene reaction. A CD spectrum shows the structural integrity of the biohybrid catalyst. MALDI-TOF of the segment containing the anchoring site for the cobalt complex Cys545 confirmed successful conjugation. This biohybrid catalyst catalyzed the cyclotrimerization of phenylacetylene to give a mixture of regioisomeric 1,2,4- and 1,3,5-triphenylbenzene in aqueous medium.

中文翻译：

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嵌入在跨膜蛋白FhuA工程变体中的钴半三明治复合物催化苯乙炔的环三聚化†

的（η ⁵ -环戊二烯基）合钴（我）配合物共价结合到跨膜蛋白的工程变体铁氧肟酸盐摄取的蛋白质组分：甲，的FhuAΔCVF ^TEV，使用硫醇-烯反应。CD谱显示了生物杂化催化剂的结构完整性。包含钴复合物Cys545锚定位点的片段的MALDI-TOF证实成功缀合。该生物杂化催化剂催化苯乙炔的环三聚反应，以在水性介质中得到区域异构的1,2,4-和1,3,5-三苯苯的混合物。