Since that pathogenic bacteria are major threats to human health, this paper describes the fabrication of an effective and durable sensing platform based on gold nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite (AuNPs/CNPs/CNFs) at the surface of glassy carbon electrode for sensitive and selective detection of Staphylococcus aureus (S. aureus). The AuNPs/CNPs/CNFs nanocomposite with the high surface area, excellent conductivity, and good biocompatibility was used for self-assembled of the thiolated specific S. aureus aptamer as a sensing element. The surface morphology of AuNPs/CNPs/CNFs nanocomposite was characterized with field emission scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), dynamic light scattering (DLS) and ultraviolet-visible (UV–Vis) spectrophotometric methods. Each aptasensor modification step was monitored with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. The fabricated aptasensor exhibited a wide linear dynamic range (1.2 × 10¹ to 1.2 × 10⁸) CFU mL⁻¹ with a LOD of 1 CFU mL⁻¹ and was be capable to accurate detection and determination of Staphylococcus aureus in human blood serum as a clinical sample with a complex matrix.

由于致病细菌是对人类健康的主要威胁，因此本文描述了一种有效且持久的传感平台的制造，该平台基于玻璃纳米碳电极表面的金纳米颗粒/碳纳米颗粒/纤维素纳米纤维纳米复合材料（AuNPs / CNPs / CNFs）。和的选择性检测金黄色葡萄球菌（小号。金黄色葡萄球菌）。具有高的表面积，优异的导电性和良好的生物相容性的AuNPs / CNPs / CNFs纳米复合材料被用于硫醇化的S的自组装。金黄色适体作为传感元件。AuNPs / CNPs / CNFs纳米复合材料的表面形态通过场发射扫描电子显微镜（FESEM），能量色散光谱（EDS），动态光散射（DLS）和紫外可见（UV-Vis）分光光度法进行了表征。每个适体传感器的修饰步骤均通过循环伏安法（CV）和电化学阻抗谱（EIS）技术进行监控。所制备的适体传感器具有宽的线性动态范围（1.2×10 ¹到1.2×10 ⁸）CFU mL ^-1，LOD为1 CFU mL ^-1，并且能够准确检测和测定人血清中的金黄色葡萄球菌。具有复杂基质的临床样品。