A new conductometric biosensor based on coimmobilized urease and arginase has been developed for arginine determination in pharmaceutics. First, the main parameters of the selected method of immobilization (concentrations of arginase, urease, and glutaraldehyde, time of incubation) were optimized. An influence of the solution parameters (buffer ionic strength, capacity, pH, Mn²⁺ concentration) on the biosensor operation was studied, working conditions were optimized. After biosensor optimization, the main analytical characteristics were as follows. The limit of detection - 2.5 μM, the linear range - 2.5–500 μM, the sensitivity to arginine 13.4 ± 2.4 μS/mM, the response time - 20 s. The signals repeatability and operational stability in continuous exploitation were studied over one working day and during one week. Additionally, the selectivity of the developed biosensor towards arginine was essayed relative to other amino acids.

The developed biosensor has been used to measure arginine concentrations in some drugs. The results obtained were in high correlation with the characteristics declared by producers.

已经开发了一种基于共固定脲酶和精氨酸酶的新型电导式生物传感器，用于测定药物中的精氨酸。首先，优化所选固定方法的主要参数（精氨酸酶，脲酶和戊二醛的浓度，孵育时间）。溶液参数的影响（缓冲液离子强度，容量，pH，Mn ²⁺对生物传感器的操作进行了研究，优化了工作条件。经过生物传感器的优化，主要分析特征如下。检测限-2.5μM，线性范围-2.5–500μM，对精氨酸的敏感性13.4±2.4μS/ mM，响应时间-20 s。在一个工作日和一个星期内研究了连续开采中的信号重复性和操作稳定性。另外，还提出了相对于其他氨基酸，已开发的生物传感器对精氨酸的选择性。

先进的生物传感器已用于测量某些药物中的精氨酸浓度。获得的结果与生产者宣称的特征高度相关。