Lipidic cubic phase systems (LCPs) are excellent carriers for immobilized enzymes due to their biocompatibility and well-defined nanoporous structure. Lipidic cubic phases act as a convenient matrix to incorporate enzymes and hold them in the vicinity of electrode surfaces in their fully active forms. Corynascus thermophilus cellobiose dehydrogenase (CtCDH) was trapped in a monoolein cubic phase, which increased not only its stability, but also its catalytic performance with both enhanced mediated and direct electron transfer with electrodes. For studies of mediated electron transfer, three mediators with different formal potentials (E°′) were employed: horse-heart cytochrome c (cyt c), electron acceptor active with the cytochrome domain of CtCDH, and 2,6-dichlorophenolindophenol (DCPIP) as well as hexaammineruthenium(II) chloride [Ru(NH₃)Cl₂] both electron acceptors with the dehydrogenase domain. Ru(NH₃)Cl₂, having the most negative E°′ of − 0.138 V vs. Ag | AgCl at pH 7.5, gave a catalytic current for lactose oxidation of 32.10 μA cm^− 2 in MOPS buffer at pH 7.5. The process carried out in the same solution but under direct electron conditions transfer resulted in a catalytic current of 9.22 μA cm^− 2. Electrodes covered with CtCDH in a LCP film retained their catalytic activity after 28 days showing a slightly increased current density after 6 days.

脂质立方相系统（LCP）由于其生物相容性和明确的纳米孔结构，是固定化酶的极佳载体。脂质立方相充当掺入酶并将其以其完全活性形式保持在电极表面附近的便利基质。嗜热Corynascus嗜热纤维二糖脱氢酶（Ct CDH）被困在单油精立方相中，这不仅增加了它的稳定性，而且还增强了其催化性能，同时增强了电极介导的和直接的电子转移。为了研究介导的电子转移，采用了三种具有不同形式势（E°'）的介体：马心细胞色素c（cyt c），具有Ct CDH细胞色素结构域的电子受体和2,6-二氯苯酚吲哚酚（DCPIP）以及氯化六氨合钌（II）[Ru（NH ₃）Cl ₂ ]都是具有脱氢酶结构域的电子受体。的Ru（NH ₃）氯₂，具有最负的E 0' - 0.138 V VS。银| 在pH 7.5的AgCl溶液中，在pH 7.5的MOPS缓冲液中产生32.10μAcm ^-2的乳糖氧化催化电流。在相同的溶液中但在直接电子条件下进行转移的过程产生了9.22μAcm ^-2的催化电流。电极被Ct覆盖LCP膜中的CDH在28天后仍保持其催化活性，显示6天后电流密度略有增加。