A Fluorescent Probe for Early Detection of Melanoma and Its Metastasis by Specifically Imaging Tyrosinase Activity in a Mouse Model,Analytical Chemistry

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A Fluorescent Probe for Early Detection of Melanoma and Its Metastasis by Specifically Imaging Tyrosinase Activity in a Mouse Model
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-07-05 00:00:00 , DOI: 10.1021/acs.analchem.8b00594
Chenyue Zhan ₁ , Jiatian Cheng ₁ , Bowen Li ₁ , Shuailing Huang ₁ , Fang Zeng ₁ , Shuizhu Wu ₁

Affiliation

Melanoma is a type of highly malignant and metastatic skin cancer, and early detection of melanoma by analyzing the level of its biomarker may decrease the likelihood of mortality. In this study, a fluorescent probe called NBR-AP for detecting tyrosinase (a biomarker of melanoma) has been created by incorporating a hydroxyphenylurea group (as a substrate for the enzyme) onto a fluorescent dye phenoxazine derivative (as an activatable signal reporter). This probe can be activated to generate fluorescence through a tyrosinase-mediated oxidation followed by hydrolysis of the urea linkage. The probe is able to detect the endogenous tyrosinase level in live cells and in zebrafish sensitively and selectively. Moreover, by imaging the tyrosinase activity, NBR-AP has been successfully applied to diagnose the melanoma and its metastasis in xenogeneic mouse models established via subcutaneous injection of B16F10 cells.

中文翻译：

一种荧光探针，用于早期检测黑色素瘤及其转移，具体通过小鼠模型中的酪氨酸酶活性成像

黑色素瘤是一种高度恶性和转移性皮肤癌，通过分析其生物标志物的水平来早期发现黑色素瘤可降低死亡的可能性。在这项研究中，通过将羟基苯基脲基团（作为酶的底物）掺入荧光染料吩恶嗪衍生物（作为可激活的信号报告物）上，创建了一种称为NBR-AP的荧光探针，用于检测酪氨酸酶（黑色素瘤的生物标志物）。该探针可以被激活，通过酪氨酸酶介导的氧化反应产生荧光，然后水解尿素键。该探针能够灵敏和选择性地检测活细胞和斑马鱼中的内源性酪氨酸酶水平。此外，通过对酪氨酸酶活性进行成像，

更新日期：2018-07-05

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