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A Dual-Response Fluorescent Probe for the Detection of Viscosity and H2S and Its Application in Studying Their Cross-Talk Influence in Mitochondria
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-07-05 00:00:00 , DOI: 10.1021/acs.analchem.8b02068 Song-Jiao Li 1 , Yong-Fei Li 1, 2 , Hong-Wen Liu 1 , Dong-Ye Zhou 1 , Wen-Li Jiang 1 , Juan Ou-Yang 1 , Chun-Yan Li 1, 3
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-07-05 00:00:00 , DOI: 10.1021/acs.analchem.8b02068 Song-Jiao Li 1 , Yong-Fei Li 1, 2 , Hong-Wen Liu 1 , Dong-Ye Zhou 1 , Wen-Li Jiang 1 , Juan Ou-Yang 1 , Chun-Yan Li 1, 3
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Intracellular viscosity is an essential microenvironmental parameter and H2S is a critical gaseous signaling molecule, which are both related to various physiological processes. It is reported that the change of viscosity and an imbalance of H2S production in the mitochondria are both associated with overexpression of amyloid betapeptide (Aβ), which is thought to play a central role in the pathogenesis of Alzheimer’s disease (AD). However, to our best knowledge, no fluorescent probe is found for dual detection of mitochondrial viscosity and H2S. Herein, a dual-response fluorescent probe (Mito-VS) is designed and synthesized to monitor the level of viscosity and H2S, respectively. Mito-VS itself is nonfluorescent due to a free intramolecular rotation between dimethylaniline and pyridine. After the increase of viscosity, the rotation is prohibited and an intense red fluorescence is released. Upon the addition of H2S, the probe can react with H2S to form compound 3 and a strong green fluorescence can be observed. Moreover, the probe possesses a good mitochondrion-targeting ability and is applied for imaging the change of viscosity on the red channel and visualizing the variation of exogenous and endogenous H2S concentration on the green channel in mitochondria. Most importantly, the probe is capable of studying the cross-talk influence of viscosity and H2S in mitochondria, which is very beneficial for knowing the pathogenesis of AD.
中文翻译：
一种用于检测粘度和H 2 S的双响应荧光探针及其在研究线粒体交叉谈话影响中的应用
细胞内粘度是必不可少的微环境参数，H 2 S是重要的气态信号分子，两者均与各种生理过程有关。据报道，线粒体中粘度的变化和H 2 S产生的失衡均与淀粉样蛋白β肽（Aβ）的过表达有关，这被认为在阿尔茨海默氏病（AD）的发病机理中起着重要作用。然而，据我们所知，没有发现用于双重检测线粒体粘度和H 2 S的荧光探针。在此，设计并合成了双响应荧光探针（Mito-VS）来监测粘度和H 2的水平。S分别。由于二甲基苯胺和吡啶之间的分子内自由旋转，所以Mito-VS本身是不发荧光的。粘度增加后，禁止旋转，并释放出强烈的红色荧光。加入H 2 S后，探针可以与H 2 S反应形成化合物3，并且可以观察到强烈的绿色荧光。此外，该探针具有良好的线粒体靶向能力，可用于对红色通道上的粘度变化进行成像，并可视化线粒体绿色通道上外源性和内源性H 2 S浓度的变化。最重要的是，该探头能够研究粘度和H 2的串扰影响线粒体中的S，对于了解AD的发病机理非常有益。
更新日期：2018-07-05
中文翻译：
一种用于检测粘度和H 2 S的双响应荧光探针及其在研究线粒体交叉谈话影响中的应用
细胞内粘度是必不可少的微环境参数，H 2 S是重要的气态信号分子，两者均与各种生理过程有关。据报道，线粒体中粘度的变化和H 2 S产生的失衡均与淀粉样蛋白β肽（Aβ）的过表达有关，这被认为在阿尔茨海默氏病（AD）的发病机理中起着重要作用。然而，据我们所知，没有发现用于双重检测线粒体粘度和H 2 S的荧光探针。在此，设计并合成了双响应荧光探针（Mito-VS）来监测粘度和H 2的水平。S分别。由于二甲基苯胺和吡啶之间的分子内自由旋转，所以Mito-VS本身是不发荧光的。粘度增加后，禁止旋转，并释放出强烈的红色荧光。加入H 2 S后，探针可以与H 2 S反应形成化合物3，并且可以观察到强烈的绿色荧光。此外，该探针具有良好的线粒体靶向能力，可用于对红色通道上的粘度变化进行成像，并可视化线粒体绿色通道上外源性和内源性H 2 S浓度的变化。最重要的是，该探头能够研究粘度和H 2的串扰影响线粒体中的S，对于了解AD的发病机理非常有益。
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