Poly(ADP-ribose) polymerase-1 (PARP-1) monitoring has attracted extensive attention because it serves a vital role in human pathologies. However, only a few researches about its detection methods have been reported because PARP-1 and its catalyzed product poly(ADP-ribose) polymer (PAR) are lack of optical or electrochemical activity. Herein, a convenient fluorescence “turn-off-on” nanosensor based on cationic conjugated polymer (PFP) and MnO₂ nanosheets has been designed for selective detection of PARP-1 in vitro. To the best of our knowledge, it is the first time to use manganese dioxide (MnO₂) nanosheets for PARP-1 detection. The fluorescence intensity of PFP can be quenched by MnO₂ nanosheets via a fluorescence resonance energy transfer (FRET). While the subsequently joined electronegative poly(ADP-ribose) polymer (PAR), catalysate of PARP-1, take positively charged MnO₂ nanosheets away from PFP via electrostatic interaction, causing sufficient recovery of fluorescent signal. The sensing platform displayed a sensitive response to PARP-1 in a linear range of 0.03 − 1.5 U (0.024 − 1.2 nM), with a detection limit of 0.004 U (0.003 nM), which is 10–100 lower than reported methods. As expected, this method was successfully applied to the detection of PARP-1 in human serum samples with recoveries ranging from 97.6 − 102.7%. Especially, it has also been applied to the determination of PARP-1 in human breast cancer cells SK-BR-3 in the 40 to 1000 cells per mL range, with a detection limit as low as 25 cells per mL.

聚（ADP-核糖）聚合酶-1（PARP-1）监测已引起广泛关注，因为它在人类病理学中起着至关重要的作用。然而，由于PARP-1及其催化产物聚（ADP-核糖）聚合物（PAR）缺乏光学或电化学活性，因此仅报道了少量有关其检测方法的研究。本文中，已经设计了一种基于阳离子共轭聚合物（PFP）和MnO ₂纳米片的方便的荧光“关闭”纳米传感器，用于体外选择性检测PARP-1。据我们所知，这是第一次使用二氧化锰（MnO ₂）纳米片进行PARP-1检测。可以用MnO ₂猝灭PFP的荧光强度纳米片通过荧光共振能量转移（FRET）。随后加入的带负电的聚（ADP-核糖）聚合物（PAR），PARP-1的催化剂，带正电的MnO ₂纳米片通过静电相互作用远离PFP，导致荧光信号充分恢复。传感平台在0.03 − 1.5 U（0.024 − 1.2 nM）的线性范围内显示出对PARP-1的敏感响应，检测限为0.004 U（0.003 nM），比报道的方法低10–100。如预期的那样，该方法已成功应用于人血清样品中PARP-1的检测，回收率为97.6-102.7％。尤其是，它也已用于测定人乳腺癌细胞SK-BR-3中PARP-1的含量，检测范围为每毫升40至1000个细胞，检出限低至每毫升25个细胞。