Voltammetric Sensor with Replaceable Polyaniline‐DNA Layer for Doxorubicin Determination,Electroanalysis

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Voltammetric Sensor with Replaceable Polyaniline‐DNA Layer for Doxorubicin Determination
Electroanalysis ( IF 2.7 ) Pub Date : 2018-07-04 , DOI: 10.1002/elan.201800331
T. N. Kulikova ₁ , A. V. Porfireva ₁ , R. V. Shamagsumova ₁ , G. A. Evtugyn ₁

Affiliation

New voltammetric DNA sensor has been developed on the base of glassy carbon electrode covered with electropolymerized polyaniline with entrapped native DNA saturated with Methylene blue. The thickness and redox properties of the coating are easily regulated by the number of potential cycles and pH of the solution. Doxorubicin competes with Methylene blue for DNA binding sites and suppresses the electron transfer within the layer. The measurement of the decay of the cathodic peak current made it possible to determine down to 0.01 nM doxorubicin. After that, DNA can be replaced by consecutive treatment of the biosensor with concentrated HCl and fresh DNA solution. Second involvement of DNA was confirmed by electrochemical impedance spectroscopy. The DNA sensor developed was tested on artificial samples mimicking ionic content of human serum and on commercial drug formulation containing doxorubicin.

中文翻译：

带有可替换聚苯胺-DNA层的伏安传感器，用于阿霉素的测定

在覆盖有电聚合聚苯胺的玻璃碳电极的基础上，开发了一种新型伏安DNA传感器，该电极具有被亚甲基蓝饱和的截留天然DNA。涂层的厚度和氧化还原特性很容易通过电位循环次数和溶液的pH值进行调节。阿霉素与亚甲蓝竞争DNA结合位点，并抑制该层内的电子转移。通过测量阴极峰值电流的衰减，可以确定低至0.01 nM的阿霉素。之后，可以通过用浓盐酸和新鲜的DNA溶液连续处理生物传感器来替换DNA。DNA的第二次介入已通过电化学阻抗光谱法确认。

更新日期：2018-07-04

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