HyPR-MS for Multiplexed Discovery of MALAT1, NEAT1, and NORAD lncRNA Protein Interactomes,Journal of Proteome Research

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HyPR-MS for Multiplexed Discovery of MALAT1, NEAT1, and NORAD lncRNA Protein Interactomes
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2018-07-31 , DOI: 10.1021/acs.jproteome.8b00189
Michele Spiniello ₁ , Rachel A. Knoener ₁ , Maisie I. Steinbrink _{1,

2} , Bing Yang ₃ , Anthony J. Cesnik ₁ , Katherine E. Buxton ₁ , Mark Scalf ₁ , David F. Jarrard _{2,

3,

4} , Lloyd M. Smith _{1,

5}

Affiliation

RNA–protein interactions are integral to the regulation of gene expression. RNAs have diverse functions and the protein interactomes of individual RNAs vary temporally, spatially, and with physiological context. These factors make the global acquisition of individual RNA–protein interactomes an essential endeavor. Although techniques have been reported for discovery of the protein interactomes of specific RNAs they are largely laborious, costly, and accomplished singly in individual experiments. We developed HyPR-MS for the discovery and analysis of the protein interactomes of multiple RNAs in a single experiment while also reducing design time and improving efficiencies. Presented here is the application of HyPR-MS to simultaneously and selectively isolate the interactomes of lncRNAs MALAT1, NEAT1, and NORAD. Our analysis features the proteins that potentially contribute to both known and previously undiscovered roles of each lncRNA. This platform provides a powerful new multiplexing tool for the efficient and cost-effective elucidation of specific RNA–protein interactomes.

中文翻译：

HyPR-MS用于发现MALAT1，NEAT1和NORAD lncRNA蛋白相互作用组的多重发现

RNA与蛋白质的相互作用是基因表达调控所必需的。RNA具有多种功能，单个RNA的蛋白质相互作用组在时间，空间和生理环境上均会发生变化。这些因素使得在全球范围内获取单个RNA-蛋白质相互作用组成为必不可少的工作。尽管已经报道了发现特定RNA的蛋白质相互作用基因组的技术，但是它们在单个实验中主要是费力，昂贵且单独完成的。我们开发了HyPR-MS，用于在单个实验中发现和分析多个RNA的蛋白质相互作用组，同时还减少了设计时间并提高了效率。本文介绍了HyPR-MS在同时并选择性分离lncRNA MALAT1，NEAT1和NORAD的相互作用基因组中的应用。我们的分析特征是可能对每种lncRNA的已知和先前未发现的作用都有贡献的蛋白质。该平台提供了功能强大的新型多路复用工具，可高效，经济高效地阐明特定的RNA-蛋白质相互作用组。

更新日期：2018-07-31

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