ESIPT-based fluorescence probe for the rapid detection of hypochlorite (HOCl/ClO−)†,Chemical Communications

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ESIPT-based fluorescence probe for the rapid detection of hypochlorite (HOCl/ClO−)†
Chemical Communications ( IF 4.3 ) Pub Date : 2018-07-04 00:00:00 , DOI: 10.1039/c8cc03717e
Luling Wu _{1,

2,

3,

4} , Qingye Yang _{1,

5,

6,

7,

8} , Liyuan Liu _{1,

2,

3,

4} , Adam C. Sedgwick _{1,

2,

3,

4} , Alexander J. Cresswell _{1,

2,

3,

4} , Steven D. Bull _{1,

2,

3,

4} , Chusen Huang _{1,

5,

6,

7,

8} , Tony D. James _{1,

2,

3,

4,

9}

Affiliation

ESIPT-based fluorescence probes are emerging as an attractive tool for the detection of biologically relevant analytes owing to their unique photophysical properties. In this work, we have developed an ESIPT-based fluorescence probe (TCBT-OMe) for the detection of HClO/ClO⁻ through the attachment of a bioorthogonal dimethylthiocarbamate linker. TCBT-OMe was shown to rapidly detect HClO/ClO⁻ (<10 s) at biologically relevant concentrations (LoD = 0.16 nM) and have an excellent selectivity towards others ROS/RNS and amino acids. Therefore, TCBT-OMe was tested in live cells and was successfully shown to be able to detect endogenous and exogenous HClO/ClO⁻ in HeLa cells. Additionally, TCBT-OMe acts as a dual input logic gate for Hg²⁺ and H₂O₂. Interestingly, Hg²⁺ alone gradually causes a fluorescence response but requires >30 min to produce a fluorescence response. Test strips containing TCBT-OMe were prepared and were demonstrated as an effective way to detect HClO/ClO⁻ in water. Furthermore, TCBT-OMe was shown to detect exogenously added HClO/ClO⁻ in three different water samples with little interference thus demonstrating the effectiveness as a method for the detection of HClO/ClO⁻ in drinking water samples.

中文翻译：

的快速检测次氯酸盐（次氯酸/ CLO的基于ESIPT荧光探针^-）†

基于ESIPT的荧光探针由于其独特的光物理特性，正成为一种有吸引力的工具，用于检测生物学上相关的分析物。在这项工作中，我们开发了一种基于ESIPT荧光探针（TCBT-OME），用于检测高氯酸/ CLO的^-通过生物正交甲基硫代氨基甲酸酯接头的附接。TCBT-OMe在生物学上相关的浓度（LoD = 0.16 nM）下可快速检测HClO / ClO ^-（<10 s），并且对其他ROS / RNS和氨基酸具有极好的选择性。因此，TCBT-OME在活细胞进行了测试，并成功地显示出能够检测内源性和外源性的HClO / CLO ^-在HeLa细胞中。此外，TCBT-OMe用作Hg ²⁺和H ₂ O ₂的双输入逻辑门。有趣的是，单独的Hg ^2+会逐渐引起荧光反应，但需要> 30分钟才能产生荧光反应。含有测试条TCBT-OME中制备和进行了论证，以检测的HClO / CLO的有效方法^-在水中。此外，TCBT-OME显示出检测外源添加的HClO / CLO ^-与如此小干扰证明效力，作为用于检测的HClO / CLO的方法在三种不同的水样^-饮用水中的样品。

更新日期：2018-07-04

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