Comparative metabolomic analysis reveals global cadmium stress response of Lactobacillus plantarum strains†,Metallomics

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Comparative metabolomic analysis reveals global cadmium stress response of Lactobacillus plantarum strains†
Metallomics ( IF 2.9 ) Pub Date : 2018-07-04 00:00:00 , DOI: 10.1039/c8mt00095f
Qixiao Zhai _{1,

2,

3,

4,

5} , Yue Xiao _{1,

2,

3,

4,

5} , Arjan Narbad _{3,

6,

7,

8,

9} , Wei Chen _{1,

2,

3,

4,

5}

Affiliation

Our previous work demonstrated the protective effects of Lactobacillus plantarum (L. plantarum) strains against cadmium (Cd) toxicity in vivo, and also indicated that the Cd tolerance of the strains played an important role in this protection. The goal of this study was to investigate the Cd resistance mechanism of L. plantarum by liquid chromatography-mass spectrometry (LC-MS) based metabolomic analysis, with a focus on the global Cd stress response. L. plantarum CCFM8610 (strongly resistant to Cd) and L. plantarum CCFM191 (sensitive to Cd) were selected as target strains, and their metabolomic profiles with and without Cd exposure were compared. The underlying mechanisms of the intra-species distinction between CCFM8610 and CCFM191 in terms of Cd tolerance can be attributed to the following aspects: (a) CCFM8610 possesses a higher intracellular content of osmolytes; (b) CCFM8610 can induce more effective biosynthesis of extracellular polymeric substance (EPS) to sequestrate Cd; (c) CCFM8610 can maintain a relatively stable nucleic acid metabolism to ensure normal growth of the strain upon Cd stress; (d) CCFM8610 exhibits a specific energy conservation mode to alleviate Cd-induced oxidative stress and/or adapt to the enhanced biosynthesis of EPS; (e) CCFM8610 has a more rigid cell membrane structure with a lower fluidity that can inhibit Cd influx into the cytoplasm.

中文翻译：

比较代谢组学分析揭示了植物乳杆菌菌株的总体镉胁迫响应†

我们以前的工作证明了植物乳杆菌（L. plantarum）菌株在体内对镉（Cd）毒性的保护作用，并且还表明该菌株对Cd的耐受性在这种保护中起着重要作用。这项研究的目的是通过基于液相色谱-质谱（LC-MS）的代谢组学分析来研究植物乳杆菌对Cd的抗性机制，重点是对整体Cd胁迫的响应。植物乳杆菌CCFM8610（对Cd有较强的抵抗力）和植物乳杆菌选择CCFM191（对Cd敏感）作为目标菌株，比较它们在有和没有Cd暴露的情况下的代谢组学特征。CCFM8610和CCFM191在Cd耐受性方面的种内差异的潜在机制可归因于以下几个方面：（a）CCFM8610具有较高的细胞内渗透压含量；（b）CCFM8610可以更有效地生物合成胞外聚合物（EPS）以螯合Cd；（c）CCFM8610可以维持相对稳定的核酸代谢，以确保在Cd胁迫下菌株的正常生长；（d）CCFM8610表现出一种特定的节能模式，以减轻Cd诱导的氧化应激和/或适应EPS增强的生物合成；

更新日期：2018-07-04

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