Bacterial community isolated from different units of a Drinking Water Treatment Plant (DWTP) including pre-ozonation unit (POU), the effluent-sludge mixture of the sedimentation unit (ESSU) and top-sand layer water sample from the filtration unit (TSFU) were acclimatized separately in the microcystin-leucine arginine (MC-LR)-rich environment to evaluate MC-LR biodegradation. Maximum biodegradation efficiency of 97.2 ± 8.7% was achieved by the acclimatized-TSFU bacterial community followed by 72.1 ± 6.4% and 86.2 ± 7.3% by acclimatized-POU and acclimatized-ESSU bacterial community, respectively. Likewise, the non-acclimatized bacterial community showed similar biodegradation efficiency of 71.1 ± 7.37%, 86.7 ± 3.19% and 94.35 ± 10.63% for TSFU, ESSU and POU, respectively, when compared to the acclimatized ones. However, the biodegradation rate increased 1.5-folds for acclimatized versus non-acclimatized conditions. The mass spectrometry studies on MC-LR degradation depicted hydrolytic linearization of cyclic MC-LR along with the formation of small peptide fragments including Adda molecule that is linked to the reduced toxicity (qualitative toxicity analysis). This was further confirmed quantitatively by using Rhizobium meliloti as a bioindicator. The acclimatized-TSFU bacterial community comprised of novel MC-LR degrading strains, Chryseobacterium sp. and Pseudomonas fragi as confirmed by 16S rRNA sequencing.

从饮用水处理厂（DWTP）的不同单元中分离出的细菌群落，包括预臭氧化单元（POU），沉淀单元的废水污泥混合物（ESSU）和来自过滤单元（TSFU）的顶层砂层水样品在富含微囊藻亮氨酸精氨酸（MC-LR）的环境中分别进行驯化，以评估MC-LR的生物降解。驯化的TSFU细菌群落的最大生物降解效率为97.2±8.7％，其次是驯化的POU和驯化的ESSU细菌群落的最大生物降解效率为72.1±6.4％和86.2±7.3％。同样，与适应环境相比，未适应的细菌群落对TSFU，ESSU和POU的生物降解效率分别为71.1±7.37％，86.7±3.19％和94.35±10.63％。然而，适应环境与非适应环境相比，生物降解率提高了1.5倍。关于MC-LR降解的质谱研究显示了环状MC-LR的水解线性化以及包括与降低的毒性有关的Adda分子的小肽片段的形成（定性毒性分析）。通过使用进一步定量确认苜蓿根瘤菌作为生物指示剂。适应的TSFU细菌群落由新型MC-LR降解菌株Chryseobacterium sp。组成。和莓实假单胞菌通过的16S rRNA测序证实。