As one of the most efficient and commonly used electrochemiluminescence (ECL) reagents, luminol has been paid much attention by the analysts due to its low excitation potential, simple dissolved oxygen-based coreactant ECL reaction requirement, and the widely analytical applications. However, the ECL performances of luminol on most electrode materials suffered from the lower ECL quantum yield, which limited its analytical applications. Herein, it was first found that, compared to that of the bare gold electrode, the ECL quantum yield of luminol on the 1,6-hexanedithiol hydrophobic pinhole film modified gold electrode was 3 times increased. This higher ECL quantum yield of luminol was related to the hydrophobic microenvironment on the surface of the modified electrode, which was formed from the hydrophobic carbon chains on the basis of their supramolecular interaction. On the basis of this new finding as well as the cap effect of gold nanoparticle to these pinhole gates, a highly sensitive ECL sensing scheme for microRNA was also developed.

中文翻译：

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通过化学修饰反应微环境增强鲁米诺的电化学发光

作为最有效和最常用的电化学发光（ECL）试剂之一，鲁米诺因其低激发电位，简单的基于溶解氧的共反应剂ECL反应要求以及广泛的分析应用而受到分析家的关注。但是，鲁米诺在大多数电极材料上的ECL性能受到ECL量子产率较低的困扰，从而限制了其分析应用。在此，首先发现，与裸金电极相比，在1，6-己二硫醇疏水针孔膜修饰金电极上鲁米诺的ECL量子产率提高了3倍。鲁米诺的较高ECL量子产率与修饰电极表面的疏水微环境有关，它是由疏水碳链根据其超分子相互作用形成的。基于这一新发现以及金纳米颗粒对这些针孔门的帽效应，还开发了针对microRNA的高灵敏度ECL传感方案。