Conventional assays using fiber-optic localized surface plasmon resonance (FO LSPR) sensors involve soaking the sensor in solution, which exposes the sensor to air during measurement. Although the exposure time is short, for a small sensor surface area, this can result in drying of biomolecules and rearrangement of nanoparticles caused by the surface tension. To minimize the resulting errors, FO LSPR sensor was combined with a micro fluidic channel. To verify the improved performance of the sensor chip combined with a micro fluidic channel, we conducted real-time detection of various concentrations of prostate-specific antigen (PSA). A calibration method was used to correct nonuniformity among the detected PSA results, arising from differences in sensors because of nonuniform metal nanoparticles on the sensor surface. A micro fluidic channel and calibration increased linearity and improved the sensitivity and dynamic range of PSA measurements. Additionally, the fabricated sensors were applied to detect the test samples and the measured sample concentrations were compared to actual values. Confirming the selectivity towards the target, the proposed system detected the control antigen. Finally, we used our sensor system to detect PSA in patient serum and acquired comparable results to those obtained using a commercialized method.

使用光纤局部表面等离振子共振（FO LSPR）传感器的常规测定包括将传感器浸入溶液中，这会使传感器在测量过程中暴露于空气中。尽管曝光时间很短，但是对于较小的传感器表面积，这可能会导致生物分子干燥以及由表面张力引起的纳米粒子重排。为了最大程度地减少错误，将FO LSPR传感器与微流体通道结合使用。为了验证结合微流体通道的传感器芯片的性能提高，我们对各种浓度的前列腺特异性抗原（PSA）进行了实时检测。由于传感器表面上的金属纳米颗粒不均匀，由传感器的差异引起的校准方法用于校正检测到的PSA结果之间的不均匀性。微流体通道和校准可增加线性度，并改善PSA测量的灵敏度和动态范围。另外，将制造的传感器应用于检测测试样品，并将测得的样品浓度与实际值进行比较。确认对靶标的选择性后，拟议的系统检测到了对照抗原。最后，我们使用传感器系统检测患者血清中的PSA，并获得了与使用商业化方法获得的结果相当的结果。拟议的系统检测到了对照抗原。最后，我们使用传感器系统检测患者血清中的PSA，并获得了与使用商业化方法获得的结果相当的结果。拟议的系统检测到了对照抗原。最后，我们使用传感器系统检测患者血清中的PSA，并获得了与使用商业化方法获得的结果相当的结果。