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Preparation of a Codelivery System Based on Vancomycin/Silk Scaffold Containing Silk Nanoparticle Loaded VEGF
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2018-07-03 00:00:00 , DOI: 10.1021/acsbiomaterials.8b00149 Negar Hassani Besheli 1 , Sheyda Damoogh 2 , Bahareh Zafar 3 , Fatemeh Mottaghitalab 4 , Hamidreza Motasadizadeh 5 , Fatemeh Rezaei 6 , Mohammad Ali Shokrgozar 2 , Mehdi Farokhi 2
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2018-07-03 00:00:00 , DOI: 10.1021/acsbiomaterials.8b00149 Negar Hassani Besheli 1 , Sheyda Damoogh 2 , Bahareh Zafar 3 , Fatemeh Mottaghitalab 4 , Hamidreza Motasadizadeh 5 , Fatemeh Rezaei 6 , Mohammad Ali Shokrgozar 2 , Mehdi Farokhi 2
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One of the main challenges of using biomaterials for inducing bone regeneration is the bacterial resistance before complete bone repair. Biomaterials with both antibacterial and bone regeneration properties are more promising for bone repair. In the present study, vascular endothelial growth factor (VEGF) was loaded on silk fibroin nanoparticles (SFNPs) and then embedded in silk scaffold containing vancomycin to form a dual drug release system. The chemical and physical properties of the fabricated structure were confirmed by Fourier transform infrared, scanning electron microscopy, and ζ-potential analysis. The size of spherical SFNPs was ∼92 nm. The release kinetics of vancomycin and VEGF showed that ∼99.56% of vancomycin and ∼14% of VEGF were released during 21 and 28 days, respectively. The bioactivity of VEGF was ∼75%. Disk diffusion test confirmed the ability of this drug delivery system against methicillin-resistant Staphylococcus aureus (MRSA). Moreover, expression of the endothelial markers (FLK-1, vWF, and VE-cadherin), alkaline phosphatase, and matrix mineral production were higher in VEGF loaded groups. Taken together, the results indicated that the fabricated codelivery system was able to simultaneously deliver antibiotic and angiogenic factor, which can be considered as a potential candidate for the treatment of contaminated bone injuries.
中文翻译：
基于万古霉素/丝质支架的纳米丝载人血管内皮生长因子代码传递系统的制备
使用生物材料诱导骨再生的主要挑战之一是在完全修复骨之前的细菌抵抗力。具有抗菌和骨骼再生特性的生物材料在骨骼修复方面更具前景。在本研究中，将血管内皮生长因子（VEGF）负载在丝素蛋白纳米颗粒（SFNPs）上，然后嵌入包含万古霉素的丝支架中，以形成双重药物释放系统。通过红外傅立叶变换，扫描电子显微镜和ζ电位分析证实了所制造结构的化学和物理性质。球形SFNP的大小约为92 nm。万古霉素和VEGF的释放动力学表明，分别在21天和28天内释放了约99.56％的万古霉素和约14％的VEGF。VEGF的生物活性为〜75％。金黄色葡萄球菌（MRSA）。此外，在加载VEGF的组中，内皮标志物（FLK-1，vWF和VE-钙粘着蛋白）的表达，碱性磷酸酶和基质矿物质的产生更高。两者合计，结果表明，预制的代码传递系统能够同时传递抗生素和血管生成因子，可以将其视为污染的骨损伤治疗的潜在候选者。
更新日期：2018-07-03
中文翻译：
基于万古霉素/丝质支架的纳米丝载人血管内皮生长因子代码传递系统的制备
使用生物材料诱导骨再生的主要挑战之一是在完全修复骨之前的细菌抵抗力。具有抗菌和骨骼再生特性的生物材料在骨骼修复方面更具前景。在本研究中，将血管内皮生长因子（VEGF）负载在丝素蛋白纳米颗粒（SFNPs）上，然后嵌入包含万古霉素的丝支架中，以形成双重药物释放系统。通过红外傅立叶变换，扫描电子显微镜和ζ电位分析证实了所制造结构的化学和物理性质。球形SFNP的大小约为92 nm。万古霉素和VEGF的释放动力学表明，分别在21天和28天内释放了约99.56％的万古霉素和约14％的VEGF。VEGF的生物活性为〜75％。金黄色葡萄球菌（MRSA）。此外，在加载VEGF的组中，内皮标志物（FLK-1，vWF和VE-钙粘着蛋白）的表达，碱性磷酸酶和基质矿物质的产生更高。两者合计，结果表明，预制的代码传递系统能够同时传递抗生素和血管生成因子，可以将其视为污染的骨损伤治疗的潜在候选者。
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