Mass spectra were recorded directly in situ the bioautogram, i.e., in the presence of microorganisms, bioassay medium and substrate reagent. The desorption-based direct analysis in real time mass spectrometry (DART-MS) was applied immediately after direct bioautography (DB). It turned out to be an advantageous combination, as it offered the possibility of a straightforward mass spectrometric detection of bioactive analytes within the bioautogram, and at the same time, it was discriminating microorganism cells and highly polar bioassay medium ingredients which could otherwise stress the MS system. DB-DART-MS was investigated for bioactive compounds in cosmetics using the Bacillus subtilis and Aliivibrio fischeri bioassays for detection of Gram-positive and Gram-negative antimicrobials, respectively, and the planar yeast estrogen screen for detection of estrogen-effective compounds. The influences of the three different bioassay matrices on the analyte response and DB-DART-MS performance on different layers were studied on the example of parabens in hand creams. It was shown that with increasing culture medium complexity, the ion suppression increased. As proof-of-principle, the mass spectrometric quantification at the nanogram level in situ the bioautogram was verified by comparison to HPTLC-DART-MS. The total paraben contents of hand creams 1 and 2 were 0.17–0.20% and 0.30–0.34%, respectively, depending on the method used (DB-DART-MS with two different bioassays or HPTLC-DART-MS as well as on RP_W or NP plate). In contrast to the current practice of applying the sample twice and subjecting one track to the bioassay and another to MS, the introduced hyphenation DB-DART-MS is straightforward and highly efficient.

直接在原位生物亲谱中记录质谱，即在存在微生物，生物测定培养基和底物试剂的情况下。直接生物自显影（DB）后立即应用实时质谱法（DART-MS）中基于解吸的直接分析。事实证明，这是一种有利的组合，因为它提供了直接进行质谱检测生物自体图内生物活性分析物的可能性，同时，它还能区分微生物细胞和高极性生物测定培养基成分，否则它们可能会对MS产生压力。系统。使用枯草芽孢杆菌和费氏Aliivibrio fischeri研究了DB-DART-MS中化妆品中的生物活性化合物分别用于检测革兰氏阳性和革兰氏阴性抗菌药物的生物测定，以及用于检测雌激素有效化合物的平面酵母雌激素筛选。以护手霜中的对羟基苯甲酸酯为例，研究了三种不同生物测定基质对不同层上分析物响应和DB-DART-MS性能的影响。结果表明，随着培养基复杂度的增加，离子抑制作用也随之增加。作为原理的证明，通过与HPTLC-DART-MS进行比较，验证了生物自传体在原位纳克级的质谱定量。护手霜1和2的对羟基苯甲酸酯总含量分别为0.17–0.20％和0.30–0.34％，具体取决于所使用的方法（具有两种不同生物测定方法的DB-DART-MS或HPTLC-DART-MS以及RP _W或NP板）。与当前两次应用样品，一条轨道进行生物测定，另一条轨道进行质谱分析的现行做法相反，引入的联用DB-DART-MS既简单又高效。