A melamine-based porous organic polymer (mPMF) was synthesized as solid phase extraction (SPE) adsorbent for inline SPE-high performance liquid chromatography (HPLC)-ultraviolet detector (UV) analysis of five phytohormones. Melamine contributed to the rich π-electron and N-containing triazine structure for mPMF, which could form π-π interaction and intermolecular hydrogen bond with COOH containing phytohormones. The synthesized mPMF adsorbent shows extremely high extraction efficiency for five target analytes (>80%) including salicylic acid, indole-3-acetic acid, abscisic acid, 2,4-dichlorophenoxyacetic acid and 1-naphthalene acetic acid. The factors affecting extraction of the target phytohormones were investigated and the optimized experimental conditions were established. The linear range was 0.2–100 μg/L with the limits of detection in the range of 0.06–0.11 μg/L for five target phytohormones. The developed method of mPMF-inline-SPE-HPLC-UV was applied for the analysis of trace target phytohormones in tomato and grape juice samples, and the recovery in the range of 83.1–116% and 87.2–121% was obtained for the spiked tomato and grape juice, respectively. This method has the advantages of high sensitivity and good reproducibility, and it has good application potential for the analysis of phytohormones in fruit samples. Moreover, inline analysis avoided the problems of sample pollution and sample loss, and provided a sample throughput of 5/h.

合成了一种基于三聚氰胺的多孔有机聚合物（mPMF）作为固相萃取（SPE）吸附剂，用于在线SPE-高效液相色谱（HPLC）-紫外检测器（UV）分析五个植物激素。三聚氰胺为mPMF贡献了丰富的π电子和含氮三嗪结构，可形成π-π相互作用和与分子间的氢键含有植物激素的COOH。合成的mPMF吸附剂对包括水杨酸，吲哚-3-乙酸，脱落酸，2,4-二氯苯氧基乙酸和1-萘乙酸在内的五种目标分析物（> 80％）具有极高的萃取效率。研究了影响目标植物激素提取的因素，并建立了优化的实验条件。线性范围为0.2–100μg/ L，五个目标植物激素的检出限为0.06–0.11μg/ L。mPMF-inline-SPE-HPLC-UV的开发方法用于分析番茄和葡萄汁样品中的痕量目标植物激素，加标样品的回收率在83.1–116％和87.2–121％范围内番茄汁和葡萄汁。该方法灵敏度高，重现性好，在水果样品中植物激素的分析中具有良好的应用潜力。此外，在线分析避免了样品污染和样品流失的问题，并提供了5 / h的样品通量。