Microfluidics for electrophysiology, imaging, and behavioral analysis of Hydra†,Lab on a Chip

当前位置： X-MOL 学术 › Lab Chip › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Microfluidics for electrophysiology, imaging, and behavioral analysis of Hydra†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-07-03 00:00:00 , DOI: 10.1039/c8lc00475g
Krishna N. Badhiwala _{1,

2,

3,

4} , Daniel L. Gonzales _{2,

3,

4,

5,

6} , Daniel G. Vercosa _{2,

3,

4,

5,

6} , Benjamin W. Avants _{2,

3,

4,

5} , Jacob T. Robinson _{1,

2,

3,

4,

5}

Affiliation

The nervous system of the cnidarian Hydra vulgaris exhibits remarkable regenerative abilities. When cut in two, the bisected tissue reorganizes into fully behaving animals in approximately 48 hours. Furthermore, new animals can reform from aggregates of dissociated cells. Understanding how behaviors are coordinated by this highly plastic nervous system could reveal basic principles of neural circuit dynamics underlying behaviors. However, Hydra's deformable and contractile body makes it difficult to manipulate the local environment while recording neural activity. Here, we present the first microfluidic technologies capable of simultaneous electrical, chemical, and optical interrogation of these soft, deformable organisms. Specifically, we demonstrate devices that can immobilize Hydra for hours-long simultaneous electrical and optical recording, and chemical stimulation of behaviors revealing neural activity during muscle contraction. We further demonstrate quantitative locomotive and behavioral tracking made possible by confining the animal to quasi-two-dimensional micro-arenas. Together, these proof-of-concept devices show that microfluidics provide a platform for scalable, quantitative cnidarian neurobiology. The experiments enabled by this technology may help reveal how highly plastic networks of neurons provide robust control of animal behavior.

中文翻译：

用于Hydra的电生理学，成像和行为分析的微流体†

刺id蛇的神经系统具有显着的再生能力。当一分为二时，平分的组织将在大约48小时内重组为行为完全的动物。此外，新动物可以从解离的细胞聚集体中重组。了解这种高度可塑性的神经系统如何协调行为，可以揭示潜在行为的神经回路动力学的基本原理。但是，Hydra的可变形和收缩体使其在记录神经活动时难以操纵局部环境。在这里，我们介绍了首批能够同时对这些柔软的可变形生物进行电，化学和光学询问的微流体技术。具体来说，我们演示了可以固定的设备Hydra可以进行长达数小时的同时电学和光学记录，并对行为进行化学刺激，以揭示肌肉收缩过程中的神经活动。我们进一步证明了通过将动物限制在准二维微型区域内来实现对机车和行为的定量跟踪。这些概念验证设备共同表明，微流体技术为可扩展的定量cnidarian神经生物学提供了一个平台。这项技术支持的实验可能有助于揭示神经元的高度可塑性网络如何提供对动物行为的鲁棒控制。

更新日期：2018-07-03

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11