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Preparation of a fermentation solution of grass fish bones and its calcium bioavailability in rats
Food & Function ( IF 5.1 ) Pub Date : 2018-07-03 , DOI: 10.1039/c8fo00674a Shuwei Tang 1, 2, 3, 4 , Shiyuan Dong 1, 2, 3, 4 , Ming Chen 1, 2, 3, 4 , Ruichang Gao 4, 5, 6, 7 , Shengjun Chen 4, 8, 9, 10, 11 , Yuanhui Zhao 1, 2, 3, 4 , Zunying Liu 1, 2, 3, 4 , Bowen Sun 1, 2, 3, 4
Food & Function ( IF 5.1 ) Pub Date : 2018-07-03 , DOI: 10.1039/c8fo00674a Shuwei Tang 1, 2, 3, 4 , Shiyuan Dong 1, 2, 3, 4 , Ming Chen 1, 2, 3, 4 , Ruichang Gao 4, 5, 6, 7 , Shengjun Chen 4, 8, 9, 10, 11 , Yuanhui Zhao 1, 2, 3, 4 , Zunying Liu 1, 2, 3, 4 , Bowen Sun 1, 2, 3, 4
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In this study, with grass fish bones as the substrate, after flavourzyme treatment, and fermentation with Leuconostoc mesenteroides, a fermentation solution with a high content of soluble calcium was obtained. High performance liquid chromatography and GC-MS analysis indicated that free calcium (11.29 mmol L−1) in the fermentation solution was composed of calcium lactate (3.89 mmol L−1), calcium acetate (6.21 mmol L−1), calcium amino acids and small peptide calcium. Animal experiments show that the fermentation solution of grass fish bones could promote the growth and development of calcium-deficient rats. Complex organic calcium could be well absorbed and utilized by rats so that serum calcium, alkaline phosphatase levels, femur weight and other indicators in calcium-deficient rats could be returned to normal levels. The fermentation solution of grass fish bones can avoid the waste of aquatic proteins and fish bone calcium, and it exhibited high calcium bioavailability. Therefore, the fermentation solution of grass fish bones might be used as a new efficient calcium supplement.
中文翻译：
草鱼骨发酵液的制备及其在大鼠体内的钙生物利用度
在本研究中，以草鱼骨为底物，经过风味酶处理，并用肠膜明串珠菌发酵，得到了可溶性钙含量高的发酵液。高效液相色谱和GC-MS分析表明，发酵液中的游离钙（11.29 mmol L -1）由乳酸钙（3.89 mmol L -1），乙酸钙（6.21 mmol L -1）组成），氨基酸钙和小肽钙。动物实验表明，草鱼骨的发酵液可以促进缺钙大鼠的生长发育。复杂的有机钙可以被大鼠很好地吸收和利用，从而使缺钙大鼠的血清钙，碱性磷酸酶水平，股骨重量和其他指标恢复到正常水平。草鱼骨的发酵液可避免浪费水生蛋白质和鱼骨钙，并具有较高的钙生物利用度。因此，草鱼骨的发酵液可作为一种新型的高效钙补充剂。
更新日期：2018-08-15
中文翻译：
草鱼骨发酵液的制备及其在大鼠体内的钙生物利用度
在本研究中，以草鱼骨为底物，经过风味酶处理，并用肠膜明串珠菌发酵，得到了可溶性钙含量高的发酵液。高效液相色谱和GC-MS分析表明，发酵液中的游离钙（11.29 mmol L -1）由乳酸钙（3.89 mmol L -1），乙酸钙（6.21 mmol L -1）组成），氨基酸钙和小肽钙。动物实验表明，草鱼骨的发酵液可以促进缺钙大鼠的生长发育。复杂的有机钙可以被大鼠很好地吸收和利用，从而使缺钙大鼠的血清钙，碱性磷酸酶水平，股骨重量和其他指标恢复到正常水平。草鱼骨的发酵液可避免浪费水生蛋白质和鱼骨钙，并具有较高的钙生物利用度。因此，草鱼骨的发酵液可作为一种新型的高效钙补充剂。
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