Surface properties and aggregation behavior of cationic-cationic and cationic-non-ionic mixed surfactant systems viz. Dodecylethyldimethylammonium bromide (DDAB) with a series of double chain cationic surfactants (DiDDAB, DMDTAB, and DODAB) and non-ionic surfactants (Brij 96, Tyloxapol and Tween 80) were analysed using surface tension and transmission electron microscopy (TEM). The effect of chain length of cationic surfactant and hydrophilic-lypophilic balance (HLB) prominently observed in critical aggregation (cac) value. The aqueous solubility of anti-tuberculosis drug: rifampicin (RIF) was comparatively studied by UV–vis spectroscopy in presence of formulated micelles and vesicles. RIF was significantly solubilised in aqueous medium using all the formulated aggregates. RIF is very unstable in basic medium (above pH-7) and in oxidizing media. Therefore, stability at pH-13 as well as in strong oxidising environment was monitored using UV–vis spectroscopy. To trace the locus of the drug encapsulation in the micelles/vesicles, fluorescence spectroscopy and TEM studies were carried out. Both the techniques stemmed in complimentary results and confirmed that, RIF is majorly populated at polar medium in cationic-cationic vesicles and favour to reside at hydrophobic medium of the nonionic-cationic micelles.

阳离子-阳离子和阳离子-非离子混合表面活性剂体系的表面性质和聚集行为。使用表面张力和透射电子显微镜（TEM）分析了十二烷基乙基二甲基溴化铵（DDAB）和一系列双链阳离子表面活性剂（DiDDAB，DMDTAB和DODAB）和非离子表面活性剂（Brij 96，Tyloxapol和Tween 80）。阳离子表面活性剂的链长和亲水亲油平衡（HLB）的影响在临界聚集（cac）值中得到显着观察。在配制的胶束和囊泡存在下，通过紫外-可见光谱法比较研究了抗结核药物：利福平（RIF）的水溶性。使用所有配制的骨料，RIF均显着溶解在水性介质中。RIF在碱性介质（pH-7以上）和氧化介质中非常不稳定。因此，使用紫外可见光谱仪可以监测pH-13以及在强氧化环境下的稳定性。为了追踪胶束/囊泡中药物包封的位置，进行了荧光光谱和TEM研究。两种技术都产生了互补的结果，并证实了RIF主要分布在阳离子阳离子囊泡的极性介质中，并且倾向于驻留在非离子阳离子胶束的疏水介质中。