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In vitro α -amylase and pancreatic lipase inhibitory activity of Cornus mas L. and Cornus alba L. fruit extracts
Journal of Food and Drug Analysis ( IF 2.6 ) Pub Date : 2019-01-01 , DOI: 10.1016/j.jfda.2018.06.005 Anita Świerczewska 1 , Tina Buchholz 2 , Matthias F Melzig 2 , Monika E Czerwińska 1
Journal of Food and Drug Analysis ( IF 2.6 ) Pub Date : 2019-01-01 , DOI: 10.1016/j.jfda.2018.06.005 Anita Świerczewska 1 , Tina Buchholz 2 , Matthias F Melzig 2 , Monika E Czerwińska 1
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Less-common fruits from Cornus spp. (Cornaceae), also named dogwoods, have shown antidiabetic, antibacterial and anti-allergic properties and are thus considered a source of phytochemicals that are beneficial to human health. The study aimed to compare the chemical compositions of the aqueous and ethanolic extracts of lyophilized fresh-picked and commercially available dried fruits of Cornus mas (Cm, cornelian cherry) and Cornus alba (Ca) fruits using HPLC-DAD-MS/MS method. Simultaneously, the α-amylase and pancreatic lipase (PL) inhibitory activities of the prepared extracts were compared by in vitro fluorescence assay based on the kinetic hydrolysis of starch or oleate ester of 4-methylumbelliferone (MUO), respectively. Additionally, a bio-assay guided identification of compounds potentially responsible for the inhibition of pancreatic enzymes was performed. Iridoids (loganic acid, cornuside) and anthocyanins (pelargonidin 3-O-galactoside) were identified in the Cm fruit extracts. Flavonoids, such as quercetin and kaempferol derivatives, were detected in the Ca fruit extracts. The chromatographic separation of the constituents of Ca fruit provided a fraction containing phenolic acids derivatives, which inhibited PL activity by 69.9 ± 4.5% at a concentration of 7.5 μg·mL-1. The IC50 of hydroxytyrosol glucoside, isolated from the most active Ca fraction, was 0.99 ± 0.10 mg·mL-1 indicating other constituents responsible for the fraction activity. The most active subfraction from Cm fruit (7.5 μg·mL-1), which inhibited PL activity by 28.3 ± 1.5%, contained pelargonidin 3-O-galactoside. Loganic acid and cornuside in highly pure form did not inhibit lipase activity. The phytochemical constituents of Cm, and particularly of Ca fruit extracts, can inhibit pancreatic enzymes and thus might be considered effective preparations in the prevention and control of hyperlipidemia related diseases.
中文翻译：
Cornus mas L.和Cornus alba L.果实提取物的体外α-淀粉酶和胰脂肪酶抑制活性
Cornus spp 中不太常见的水果。（山茱萸科），也称为山茱萸，具有抗糖尿病、抗菌和抗过敏的特性，因此被认为是对人体健康有益的植物化学物质的来源。本研究旨在使用 HPLC-DAD-MS/MS 方法比较冻干的新鲜采摘和市售的山茱萸 (Cm, 萸肉) 和白山茱萸 (Ca) 果实的水提取物和乙醇提取物的化学成分。同时，分别基于4-甲基伞形酮（MUO）的淀粉或油酸酯的动力学水解，通过体外荧光测定法比较了制备的提取物的α-淀粉酶和胰脂肪酶（PL）抑制活性。此外，对可能负责抑制胰酶的化合物进行了生物测定指导的鉴定。在 Cm 果实提取物中鉴定出环烯醚萜（loganic acid、cornuside）和花青素（pelargonidin 3-O-galactoside）。在 Ca 果实提取物中检测到黄酮类化合物，例如槲皮素和山柰酚衍生物。对钙果的成分进行色谱分离得到了含有酚酸衍生物的馏分，在7.5 μg·mL-1的浓度下，其对PL活性的抑制率为69.9 ± 4.5%。羟基酪醇糖苷的IC50，从最活跃的Ca 馏分中分离出来，为0.99 ± 0.10 mg·mL-1，表明其他成分负责该馏分的活性。Cm 果实中最活跃的亚组分（7.5 μg·mL-1），可抑制 28.3 ± 1.5% 的 PL 活性，含有天竺葵素 3-O-半乳糖苷。高纯度形式的月桂酸和山茱萸苷不抑制脂肪酶活性。Cm的植物化学成分，特别是Ca果实提取物，可以抑制胰酶，因此可能被认为是预防和控制高脂血症相关疾病的有效制剂。
更新日期：2019-01-01
中文翻译：
Cornus mas L.和Cornus alba L.果实提取物的体外α-淀粉酶和胰脂肪酶抑制活性
Cornus spp 中不太常见的水果。（山茱萸科），也称为山茱萸，具有抗糖尿病、抗菌和抗过敏的特性，因此被认为是对人体健康有益的植物化学物质的来源。本研究旨在使用 HPLC-DAD-MS/MS 方法比较冻干的新鲜采摘和市售的山茱萸 (Cm, 萸肉) 和白山茱萸 (Ca) 果实的水提取物和乙醇提取物的化学成分。同时，分别基于4-甲基伞形酮（MUO）的淀粉或油酸酯的动力学水解，通过体外荧光测定法比较了制备的提取物的α-淀粉酶和胰脂肪酶（PL）抑制活性。此外，对可能负责抑制胰酶的化合物进行了生物测定指导的鉴定。在 Cm 果实提取物中鉴定出环烯醚萜（loganic acid、cornuside）和花青素（pelargonidin 3-O-galactoside）。在 Ca 果实提取物中检测到黄酮类化合物，例如槲皮素和山柰酚衍生物。对钙果的成分进行色谱分离得到了含有酚酸衍生物的馏分，在7.5 μg·mL-1的浓度下，其对PL活性的抑制率为69.9 ± 4.5%。羟基酪醇糖苷的IC50，从最活跃的Ca 馏分中分离出来，为0.99 ± 0.10 mg·mL-1，表明其他成分负责该馏分的活性。Cm 果实中最活跃的亚组分（7.5 μg·mL-1），可抑制 28.3 ± 1.5% 的 PL 活性，含有天竺葵素 3-O-半乳糖苷。高纯度形式的月桂酸和山茱萸苷不抑制脂肪酶活性。Cm的植物化学成分，特别是Ca果实提取物，可以抑制胰酶，因此可能被认为是预防和控制高脂血症相关疾病的有效制剂。
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