Grafting through Method for Implanting of Lysozyme Enzyme in Molecular Brush for Improved Biocatalytic Activity and Thermal Stability,Macromolecules

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Grafting through Method for Implanting of Lysozyme Enzyme in Molecular Brush for Improved Biocatalytic Activity and Thermal Stability
Macromolecules ( IF 5.1 ) Pub Date : 2018-07-03 00:00:00 , DOI: 10.1021/acs.macromol.8b00991
Xue Wang ₁ , Nataraja S. Yadavalli ₁ , Amine M. Laradji ₁ , Sergiy Minko ₁

Affiliation

We report a “grafting through” conjugation strategy to improve lysozyme catalytic activity and thermal stability by the synthesis of a synthetic polymer–enzyme hybrid. The lysozyme was first conjugated with glycidyl methacrylate via ring-opening reaction between epoxy groups and lysine residues of the enzyme to synthesize the enzyme macromonomer. The conjugation was followed by free radical copolymerization of the macromonomer with poly(ethylene glycol) methyl ether acrylate (PEGMEA) (M_n = 5000 g/mol) and poly(ethylene glycol) methyl ether methacrylate (PEGMEMA) (M_n = 500 g/mol). We demonstrated that implanting of a single lysozyme molecule in the molecular brush polymer chain resulted a significant improvement of the thermal stability up to 90 °C and 9-time extended half-life for this synthetic enzyme structure. The improved enzyme performance is explained by the crowding effect provided by molecular brush architecture of the synthetic hybrid.

中文翻译：

通过在分子刷中植入溶菌酶的方法进行嫁接，以提高生物催化活性和热稳定性

我们报道了通过合成聚合物-酶杂合体的合成来改善溶菌酶催化活性和热稳定性的“嫁接”偶联策略。首先通过环氧基和酶的赖氨酸残基之间的开环反应将溶菌酶与甲基丙烯酸缩水甘油酯缀合，以合成酶大分子单体。共轭之后，大分子单体与聚（乙二醇）甲基醚丙烯酸甲酯（PEGMEA）（M _n = 5000 g / mol）和聚（乙二醇）甲基醚甲基丙烯酸甲酯（PEGMEMA）（M _n= 500 g / mol）。我们证明了在分子刷状聚合物链中植入单个溶菌酶分子可导致高达90°C的热稳定性显着改善，并且该合成酶结构的半衰期延长了9倍。合成杂种的分子刷结构提供的拥挤效应解释了酶性能的提高。

更新日期：2018-07-03

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