A Two-Photon Fluorescent Probe for Imaging Endogenous ONOO– near NMDA Receptors in Neuronal Cells and Hippocampal Tissues,Analytical Chemistry

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A Two-Photon Fluorescent Probe for Imaging Endogenous ONOO– near NMDA Receptors in Neuronal Cells and Hippocampal Tissues
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-07-03 00:00:00 , DOI: 10.1021/acs.analchem.8b01960
Dayoung Lee ₁ , Chang Su Lim ₂ , Gyeongju Ko ₁ , Dayoung Kim ₁ , Myoung Ki Cho ₂ , Sang-Jip Nam ₁ , Hwan Myung Kim ₂ , Juyoung Yoon ₁

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In this study, we developed a two-photon fluorescent probe for detection of peroxynitrite (ONOO^–) near the N-methyl-d-aspartate (NMDA) receptor. This naphthalimide-based probe contains a boronic acid reactive group and an ifenprodil-like tail, which serves as an NMDA receptor targeting unit. The probe displays high sensitivity and selectivity, along with a fast response time in aqueous solution. More importantly, the probe can be employed along with two-photon fluorescence microscopy to detect endogenous ONOO^– near NMDA receptors in neuronal cells as well as in hippocampal tissues. The results suggest that the probe has the potential of serving as a useful imaging tool for studying ONOO^– related diseases in the nervous system.

中文翻译：

两光子荧光探针成像神经元细胞和海马组织中的内源性ONOO ^– NMDA受体附近

在这项研究中，我们开发了双光子荧光探针检测过氧亚硝酸盐（ONOO ^-）附近的ñ甲基d天冬氨酸（NMDA）受体。这种基于萘二甲酰亚胺的探针包含一个硼酸反应基团和一个艾芬地尔样的尾巴，用作NMDA受体靶向单元。该探针在水溶液中显示出高灵敏度和选择性，以及快速的响应时间。更重要的是，该探针可与双光子荧光显微镜一起使用，以检测神经元细胞和海马组织中内源性ONOO ^–接近NMDA受体。结果表明，该探针具有作为研究ONOO的有用成像工具的潜力^- 神经系统中的相关疾病。

更新日期：2018-07-03

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