Base excision repair initiated rolling circle amplification-based fluorescent assay for screening uracil-DNA glycosylase activity using Endo IV-assisted cleavage of AP probes,The Analyst

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Base excision repair initiated rolling circle amplification-based fluorescent assay for screening uracil-DNA glycosylase activity using Endo IV-assisted cleavage of AP probes
The Analyst Pub Date : 2018-07-03 , DOI: 10.1039/c8an00716k
Jingfeng Wang _{1,

2,

3,

4} , Yu Wang _{1,

2,

3,

4} , Su Liu _{2,

3,

4,

5} , Haiwang Wang _{1,

2,

3,

4} , Xue Zhang _{1,

2,

3,

4} , Xiaolei Song _{2,

3,

4,

5} , Jiadong Huang _{1,

2,

3,

4,

6}

Affiliation

A simple, robust and cost effective biosensing platform for the ultrasensitive detection of UDG activity was established based on base excision repair-initiated primer generation for RCA with Endo IV-assisted signal amplification.

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