4-Hydroxy-d-phenylglycine was modified with methacrylic anhydride and then immobilized on silica through thiol-initiated surface polymerization; the prepared material was applied as stationary phase for HPLC. The obtained stationary phase was characterized by elemental analysis, infrared spectroscopy, and thermogravimetric analysis. The chromatographic performance of the packed column was evaluated in reversed-phase liquid chromatograph (RPLC) and hydrophilic interaction liquid chromatograph (HILIC) mode; this column has shown excellent selectivity to both the hydrophobic and hydrophilic solutes. The selectivity towards polycyclic aromatic hydrocarbons relative to that towards alkylbenzenes exhibited by the prepared column was higher than the corresponding selectivity exhibited by commercial C18 column, which could be explained by electronic π-π interaction between phenylglycine and electron-rich aromatic rings. On the other hand, the prepared column has also shown better selectivity for polar compounds, which was based on the multiple interaction and retention mechanisms. It was also used to separate sulfonamides and organic acid compared with a commercial C18 and HILIC column; the results show its great chromatographic performance with distinctive selectivity. All the results indicated the prepared column had potential application in a wide range.

中文翻译：

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表面键合的苯基甘氨酸两性离子固定相的制备与评价

4-羟基d用甲基丙烯酸酐对-苯基甘氨酸进行改性，然后通过硫醇引发的表面聚合将其固定在二氧化硅上；制备的物质用作HPLC的固定相。通过元素分析，红外光谱和热重分析对获得的固定相进行表征。在反相液相色谱（RPLC）和亲水作用液相色谱（HILIC）模式下对填充柱的色谱性能进行了评估；该色谱柱对疏水性和亲水性溶质均具有出色的选择性。相对于制得的色谱柱显示的对多环芳烃的选择性，高于商品化的C18色谱柱显示的相应的选择性，这可以通过苯甘氨酸和富电子芳环之间的电子π-π相互作用来解释。另一方面，基于多种相互作用和保留机制，制备的色谱柱对极性化合物的选择性也更好。与市售的C18和HILIC色谱柱相比，它还可用于分离磺酰胺和有机酸。结果表明，它具有出色的色谱性能和独特的选择性。所有结果表明，所制备的色谱柱具有广泛的应用前景。与市售的C18和HILIC色谱柱相比，它还可用于分离磺酰胺和有机酸。结果表明，它具有出色的色谱性能和独特的选择性。所有结果表明，所制备的色谱柱具有广泛的应用前景。与市售的C18和HILIC色谱柱相比，它还可用于分离磺酰胺和有机酸。结果表明，它具有出色的色谱性能和独特的选择性。所有结果表明，所制备的色谱柱具有广泛的应用前景。