Despite the wide use of 1,2,4,5- tetrazines in biomaterials and materials science, currently there does not exist synthetic method(s) that can yield significant amount of 1,2,4,5- tetrazines without the use of potentially toxic metal catalysts. Here, we report a less energy intensive and more efficient metal catalyst free approach for the synthesis of an asymmetric tetrazine. A range of operating parameters such as extraction pH and temperature were regulated to achieve a practical yield nearly 1.5 times greater than the yields reported in the literature for similar synthetic procedures.

中文翻译：

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一种高效的无金属催化剂的方法来合成5-（4-（1,2,4,5四嗪-3-基）苄氨基）-5-氧戊酸

尽管在生物材料和材料科学中广泛使用了1,2,4,5-四嗪，但目前尚不存在无需大量使用即可产生大量1,2,4,5-四嗪的合成方法。有毒金属催化剂。在这里，我们报告了不对称的四嗪合成的低能量消耗和更有效的无金属催化剂的方法。调节一系列操作参数，例如萃取pH和温度，以达到实际收率，该收率比类似合成方法文献中报道的收率高出将近1.5倍。