In designing bioassay systems for low-abundance biomolecule detection, most research focuses on improving transduction mechanisms while ignoring the intrinsically fundamental limitations in solution: mass transfer and binding affinity. We demonstrate enhanced biomolecular surface binding using an acoustic nano-electromechanical system (NEMS) resonator, as an on-chip biomolecular concentrator which breaks both mass transfer and binding affinity limitations. As a result, a concentration factor of 10⁵ has been obtained for various biomolecules. The resultantly enhanced surface binding between probes on the absorption surface and analytes in solution enables us to lower the limit of detection for representative proteins. We also integrated the biomolecular concentrator into an optoelectronic bioassay platform to demonstrate delivery of proteins from buffer/serum to the absorption surface. Since the manufacture of the resonator is CMOS-compatible, we expect it to be readily applied to further analysis of biomolecular interactions in molecular diagnostics.

中文翻译：

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基于声NEMS谐振器的通用生物分子浓缩器，可增强生物分子表面结合

在设计用于低丰度生物分子检测的生物测定系统时，大多数研究都集中在改进转导机制，而忽略了溶液的内在基本限制：传质和结合亲和力。我们展示了使用声学纳米机电系统（NEMS）共振器增强的生物分子表面结合，作为片上生物分子浓缩器，打破了质量转移和结合亲和力的局限性。结果，集中系数为10 ⁵已经获得了各种生物分子的信息。结果，吸收表面上的探针与溶液中的分析物之间增强的表面结合使我们能够降低代表性蛋白质的检测限。我们还将生物分子浓缩器集成到光电生物测定平台中，以演示蛋白质从缓冲液/血清到吸收表面的传递。由于谐振器的制造是兼容CMOS的，因此我们希望它可以很容易地应用于分子诊断中生物分子相互作用的进一步分析。