Robust approaches for chemogenetic control of protein function would have many biological applications. We developed stabilizable polypeptide linkages (StaPLs) based on hepatitis C virus protease. StaPLs undergo autoproteolysis to cleave proteins by default, whereas protease inhibitors prevent cleavage and preserve protein function. We created StaPLs responsive to different clinically approved drugs to bidirectionally control transcription with zinc-finger-based effectors, and used StaPLs to create single-chain, drug-stabilizable variants of CRISPR–Cas9 and caspase-9.

中文翻译：

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StaPLs：通用的基因编码模块，用于工程化药物诱导蛋白

化学功能控制蛋白质功能的稳健方法将有许多生物学应用。我们基于丙型肝炎病毒蛋白酶开发了可稳定的多肽键（StaPLs）。StaPLs会默认进行自动蛋白水解以裂解蛋白质，而蛋白酶抑制剂可防止裂解并保持蛋白质功能。我们创建了对Staples的反应，它们对不同的临床批准药物产生了反应，并使用基于锌指的效应子双向控制转录，并使用StaPLs创建了CRISPR–Cas9和caspase-9的单链，药物稳定的变体。