We developed a method in which the NS3 cis-protease from hepatitis C virus can be used as a ligand-inducible connection to control the function and localization of engineered proteins in mammalian cells. To demonstrate the versatility of this approach, we designed drug-sensitive transcription factors and transmembrane signaling proteins, the activities of which can be tightly and reversibly controlled through the use of clinically tested antiviral protease inhibitors.

中文翻译：

---

化学表达控制抗病毒药物的基因表达和细胞信号传导。

我们开发了一种方法，其中来自丙型肝炎病毒的NS3顺式蛋白酶可用作配体诱导的连接，以控制哺乳动物细胞中工程蛋白的功能和定位。为了证明这种方法的多功能性，我们设计了对药物敏感的转录因子和跨膜信号蛋白，可以通过使用经过临床测试的抗病毒蛋白酶抑制剂来严格且可逆地控制其活性。