A new wearable potentiometric tattoo biosensor for real-time on-body monitoring of G-type nerve agents simulant is described. The skin-worn flexible biosensor responds rapidly and selectively to the fluorine-containing organophosphates (OP) nerve agent simulant diisopropyl fluorophosphate (DFP, a model OP analyte) in both liquid and vapor phases. The epidermal potentiometric OP biosensor relies on the pH-sensitive polyaniline (PANi) coating on a flexible printed transducer for monitoring the proton release during the enzymatic hydrolysis of DFP by enzyme organophosphate hydrolase (OPH). The sensing electrodes are screen printed on a temporary tattoo paper and are interfaced to a conformal electronic interface that provides wireless data transmission. The skin-worn OP potentiometric sensor can withstand severe mechanical strains without compromising its analytical performance. The biosensor displays a wide dynamic range, fast response and high selectivity towards DFP (including efficient discrimination against organophosphate pesticides), and good reproducibility. The attractive performance of the new wearable biosensor indicates considerable promise for on-body threat detection towards rapid warning regarding potential exposure to G-series nerve agents.

描述了一种新型的可穿戴式电位纹身生物传感器，用于实时监测G型神经毒剂模拟物。皮肤佩戴式柔性生物传感器在液相和气相中均能对含氟有机磷酸酯（OP）神经毒剂模拟物二异丙基氟磷酸酯（DFP，一种OP模型分析物）做出快速而选择性的响应。表皮电位OP生物传感器依赖于柔性印刷传感器上的pH敏感聚苯胺（PANi）涂层，用于监测DFP被有机磷酸酯水解酶（OPH）酶水解过程中的质子释放。感应电极丝网印刷在临时纹身纸上，并与提供无线数据传输的保形电子接口相连接。皮肤佩戴式OP电位传感器可以承受严重的机械应力，而不会影响其分析性能。该生物传感器显示出宽广的动态范围，对DFP的快速响应和高选择性（包括对有机磷酸酯农药的有效区分）以及良好的重现性。新型可穿戴生物传感器的引人入胜的性能表明，对于威胁物潜在暴露于G系列神经毒剂的快速预警，具有很大的前景。