In Vivo Photoacoustic Detection and Imaging of Peroxynitrite,Analytical Chemistry

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In Vivo Photoacoustic Detection and Imaging of Peroxynitrite
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-07-02 00:00:00 , DOI: 10.1021/acs.analchem.8b01992
Xialing Qin ₁ , Fan Li ₁ , Yifan Zhang ₁ , Gongcheng Ma ₁ , Tao Feng ₁ , Yongxiang Luo ₁ , Peng Huang ₁ , Jing Lin ₁

Affiliation

Photoacoustic detection is an emerging noninvasive and nonionizing detection technique with the merits of rich contrast, high resolution, and deep tissue penetration, especially for in vivo detection and imaging. Herein, we developed a photoacoustic (PA) molecular imaging probe (denoted as nanonaps) composed of a naphthalocyanine dye and a heptamethine dye as the internal standard with unchanged signals at 860 nm and the sensing component with peroxynitrite (ONOO^–) target-decreased signals at 775 nm, respectively. The as-prepared nanonaps displayed high sensitivity and specificity of ONOO^– both in vitro and in vivo. The PA₈₆₀/PA₇₇₅ ratio was increased as a function of the concentration of ONOO^– (0–250 nM). More interestingly, our ratiometric nanonaps could be used for in vivo detection and imaging of ONOO^–.

中文翻译：

过氧亚硝酸盐的体内光声检测和成像

光声检测是一种新兴的非侵入性和非电离性检测技术，具有对比度高，分辨率高和组织穿透力强的优点，特别是用于体内检测和成像。在此，我们开发了一种光声（PA）分子成像探针（表示为纳米午睡），该探针由萘酞菁染料和庚二甲胺染料作为内标，在860 nm处信号不变，而在过氧亚硝酸盐（ONOO ^–）靶信号下的传感组件分别在775 nm处所制备的nanonaps显示ONOO的高灵敏度和特异性^-无论是在体外和体内。PA ₈₆₀ / PA _775的比例随ONOO浓度的增加而增加^–（0–250 nM）。更有趣的是，我们的比例纳米午睡可用于ONOO ^–的体内检测和成像。

更新日期：2018-07-02

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