Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging,ACS Chemical Biology

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Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2018-07-02 00:00:00 , DOI: 10.1021/acschembio.8b00200
Yoshiyuki Arai _{1,

2} , Hiroki Takauchi ₂ , Yuhei Ogami ₂ , Satsuki Fujiwara ₁ , Masahiro Nakano _{1,

2} , Tomoki Matsuda _{1,

2} , Takeharu Nagai _{1,

2}

Affiliation

Super-resolution imaging techniques based on single molecule localization microscopy (SMLM) broke the diffraction limit of optical microscopy in living samples with the aid of photoswitchable fluorescent probes and intricate microscopy systems. Here, we developed a fluorescent protein, SPOON, which can be switched off by excitation light illumination and switched on by thermally induced dehydration, resulting in an apparent spontaneous blinking behavior. This unique property of SPOON provides a simple SMLM-based super-resolution imaging platform which requires only a single 488 nm laser.

中文翻译：

自发闪烁的荧光蛋白，用于简单的单激光超分辨率活细胞成像

基于单分子定位显微镜（SMLM）的超分辨率成像技术借助光开关荧光探针和复杂的显微镜系统打破了活体样品中光学显微镜的衍射极限。在这里，我们开发了一种荧光蛋白SPOON，可以通过激发光照明将其关闭，并通过热诱导脱水将其打开，从而导致明显的自发闪烁行为。SPOON的这一独特特性提供了一个简单的基于SMLM的超分辨率成像平台，该平台仅需一个488 nm激光器。

更新日期：2018-07-02

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