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Nanosensing of ATP by fluorescence recovery after surface energy transfer between rhodamine B and curcubit[7]uril-capped gold nanoparticles
Microchimica Acta ( IF 5.3 ) Pub Date : 2018-07-01 , DOI: 10.1007/s00604-018-2901-8
Riham El Kurdi , Digambara Patra

AbstractThe authors describe a method for functionalization of gold nanoparticles (AuNPs) with the supramolecular host molecule, curcubit[7]uril (CB[7]) which can bind rhodamine B (RhB). The fluorescence of RhB is quenched by the AuNPs via surface energy transfer. On addition of ATP, a dimeric RhB-ATP complex is formed and RhB is pushed out of CB[7]. Hence, fluorescence increases by a factor of 8. This fluorescence recovery effect has been utilized to develop a new detection scheme for ATP. The assay, measured at fluorescence excitation and emission wavelengths of 500 nm and 574 nm respectively, works in the 0.5–10 μM concentration range and has a 100 nM detection limit. The method is not interfered by UTP, GTP, CTP, TTP, ascorbic acid and glutathione. Graphical abstractSchematic of a method for determination of ATP in the 500 nM to 10 μM concentration range by using fluorescence recovery after surface energy transfer (SET) between rhodamine B (RhB) and gold nanoparticles capped with curcubit[7]uril (CB[7]).

中文翻译:

罗丹明 B 和葫芦素 [7] 脲封端的金纳米粒子之间表面能转移后通过荧光恢复对 ATP 进行纳米传感

摘要作者描述了一种使用可结合罗丹明 B (RhB) 的超分子宿主分子 curcubit[7]uril (CB[7]) 对金纳米粒子 (AuNPs) 进行功能化的方法。RhB 的荧光通过表面能转移被 AuNPs 淬灭。加入 ATP 后,形成二聚体 RhB-ATP 复合物,RhB 被推出 CB[7]。因此,荧光增加了 8 倍。这种荧光恢复效应已被用于开发一种新的 ATP 检测方案。该测定分别在 500 nm 和 574 nm 的荧光激发和发射波长下测量,在 0.5–10 μM 浓度范围内工作,检测限为 100 nM。该方法不受 UTP、GTP、CTP、TTP、抗坏血酸和谷胱甘肽的干扰。
更新日期:2018-07-01
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