Multisensor Imaging—From Sample Preparation to Integrated Multimodal Interpretation of LA-ICPMS and MALDI MS Imaging Data,Analytical Chemistry

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Multisensor Imaging—From Sample Preparation to Integrated Multimodal Interpretation of LA-ICPMS and MALDI MS Imaging Data
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-06-30 00:00:00 , DOI: 10.1021/acs.analchem.8b00816
Matthias Holzlechner ₁ , Maximilian Bonta ₁ , Hans Lohninger ₁ , Andreas Limbeck ₁ , Martina Marchetti-Deschmann ₁

Affiliation

Laterally resolved chemical analysis (chemical imaging) has increasingly attracted attention in the Life Sciences during the past years. While some developments have provided improvements in lateral resolution and speed of analysis, there is a trend toward the combination of two or more analysis techniques, so-called multisensor imaging, for providing deeper information into the biochemical processes within one sample. In this work, a human malignant pleural mesothelioma sample from a patient treated with cisplatin as a cytostatic agent has been analyzed using laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). While LA-ICPMS was able to provide quantitative information on the platinum distribution along with the distribution of other elemental analytes in the tissue sample, MALDI MS could reveal full information on lipid distributions, as both modes of polarity, negative and positive, were used for measurements. Tandem MS experiments verified the occurrence of distinct lipid classes. All imaging analyses were performed using a lateral resolution of 40 μm, providing information with excellent depth of details. By analyzing the very same tissue section, it was possible to perfectly correlate the obtained analyte distribution information in an evaluation approach comprising LA-ICPMS and MALDI MS data. Correlations between platinum, phosphorus, and lipid distributions were found by the use of advanced statistics. The present proof-of-principle study demonstrates the benefit of data combination for outcomes beyond one method imaging modality and highlights the value of advanced chemical imaging in the Life Sciences.

中文翻译：

多传感器成像-从样品制备到LA-ICPMS和MALDI MS成像数据的集成多模式解释

在过去的几年中，横向解析化学分析（化学成像）在生命科学领域越来越受到关注。尽管一些进展已改善了横向分辨率和分析速度，但仍存在两种或多种分析技术（所谓的多传感器成像）相结合的趋势，该技术可为一个样品内的生化过程提供更深的信息。在这项工作中，使用激光消融电感耦合等离子体质谱（LA-ICPMS）和基质辅助激光解吸/电离质谱（MALDI MS）分析了以顺铂为细胞抑制剂治疗的患者的人类恶性胸膜间皮瘤样品。尽管LA-ICPMS能够提供有关组织样品中铂分布以及其他元素分析物分布的定量信息，但MALDI MS可以显示有关脂质分布的完整信息，因为极性，负和正两种模式都用于测量。串联质谱实验验证了不同脂质类别的发生。所有成像分析均使用40μm的横向分辨率进行，可提供具有出色细节深度的信息。通过分析非常相同的组织切片，可以在包括LA-ICPMS和MALDI MS数据的评估方法中完美关联获得的分析物分布信息。通过使用高级统计发现了铂，磷和脂质分布之间的相关性。

更新日期：2018-06-30

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