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Acetylated Polyethylenimine-Entrapped Gold Nanoparticles Enable Negative Computed Tomography Imaging of Orthotopic Hepatic Carcinoma
Langmuir ( IF 3.7 ) Pub Date : 2018-06-29 00:00:00 , DOI: 10.1021/acs.langmuir.8b01669 Benqing Zhou 1, 2 , Zhijuan Xiong 2 , Peng Wang 2 , Chen Peng 1 , Mingwu Shen 2 , Xiangyang Shi 1, 2, 3
Langmuir ( IF 3.7 ) Pub Date : 2018-06-29 00:00:00 , DOI: 10.1021/acs.langmuir.8b01669 Benqing Zhou 1, 2 , Zhijuan Xiong 2 , Peng Wang 2 , Chen Peng 1 , Mingwu Shen 2 , Xiangyang Shi 1, 2, 3
Affiliation
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Developing an effective computed tomography (CT) contrast agent is still a challenging task for precise diagnosis of hepatic carcinoma (HCC). Here, we present the use of acetylated polyethylenimine (PEI)-entrapped gold nanoparticles (Ac-PE-AuNPs) without antifouling modification for negative CT imaging of HCC. PEI was first linked to fluorescein isothiocyanate (FI) and then utilized as a vehicle for the entrapment of AuNPs. The particles were then acetylated to reduce its positive surface potential. The designed Ac-PE-AuNPs were characterized by various techniques. We find that the Ac-PE-AuNPs with a uniform size distribution (mean diameter = 2.3 nm) are colloidally stable and possess low toxicity in the studied range of concentration. Owing to the fact that the particles without additional antifouling modification were mainly gathered in liver, the Ac-PE-AuNPs could greatly improve the CT contrast enhancement of normal liver, whereas poor CT contrast enhancement appeared in liver necrosis region caused by HCC. As a result, HCC could be easily and precisely diagnosed. The designed Ac-PE-AuNPs were demonstrated to have biocompatibility through in vivo biodistribution and histological studies, hence holding an enormous potential to be adopted as an effective negative CT contrast agent for diagnosis of hepatoma carcinoma.
中文翻译：
乙酰化聚乙烯亚胺包裹金纳米粒子使原位肝癌的负计算机断层扫描成像。
对于精确诊断肝癌（HCC），开发有效的计算机断层扫描（CT）造影剂仍然是一项艰巨的任务。在这里，我们目前使用不带防污修饰的乙酰化聚乙烯亚胺（PEI）包裹的金纳米颗粒（Ac-PE-AuNPs）用于HCC的负CT成像。首先将PEI连接至异硫氰酸荧光素（FI），然后将其用作截留AuNP的载体。然后将颗粒乙酰化以降低其正表面电势。通过多种技术对设计的Ac-PE-AuNPs进行了表征。我们发现具有均一的尺寸分布（平均直径= 2.3 nm）的Ac-PE-AuNPs胶体稳定，并且在所研究的浓度范围内具有较低的毒性。由于没有其他防污改性的颗粒主要聚集在肝脏中，Ac-PE-AuNPs可以显着改善正常肝脏的CT对比增强，而HCC引起的肝坏死区域则显示差的CT对比增强。结果，可以容易且精确地诊断出HCC。通过体内生物分布和组织学研究证明所设计的Ac-PE-AuNP具有生物相容性，因此具有巨大的潜力，可被用作诊断肝癌的有效阴性CT造影剂。
更新日期：2018-06-29
中文翻译：
乙酰化聚乙烯亚胺包裹金纳米粒子使原位肝癌的负计算机断层扫描成像。
对于精确诊断肝癌（HCC），开发有效的计算机断层扫描（CT）造影剂仍然是一项艰巨的任务。在这里，我们目前使用不带防污修饰的乙酰化聚乙烯亚胺（PEI）包裹的金纳米颗粒（Ac-PE-AuNPs）用于HCC的负CT成像。首先将PEI连接至异硫氰酸荧光素（FI），然后将其用作截留AuNP的载体。然后将颗粒乙酰化以降低其正表面电势。通过多种技术对设计的Ac-PE-AuNPs进行了表征。我们发现具有均一的尺寸分布（平均直径= 2.3 nm）的Ac-PE-AuNPs胶体稳定，并且在所研究的浓度范围内具有较低的毒性。由于没有其他防污改性的颗粒主要聚集在肝脏中，Ac-PE-AuNPs可以显着改善正常肝脏的CT对比增强，而HCC引起的肝坏死区域则显示差的CT对比增强。结果，可以容易且精确地诊断出HCC。通过体内生物分布和组织学研究证明所设计的Ac-PE-AuNP具有生物相容性，因此具有巨大的潜力，可被用作诊断肝癌的有效阴性CT造影剂。
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