This study shows that it is possible to customize the ion-current-rectification (ICR)-based pH response in glass nanopipettes by adjusting the ratio of 3-aminopropyldimethylethoxysilane (APTES) and (3-triethoxysilylpropyl) succinic acid anhydride (TESP-SA) used in silanization. The silanization time and concentration were optimized by investigating the rectification ratio of the nanopipettes. For each APTES/TESP-SA silane ratio, analysis of variance (ANOVA) tests indicated that the rectification ratio of the nanopipette had good linearity in the pH range 3–8. Moreover, nanopipettes silanized with equal concentrations of amino and carboxylic groups gave the highest pH response of 1.06 × 10⁻⁴ pH ⁻¹. This method for customizing the ICR-based pH response in nanopipettes has the potential and versatility to be used in applications such as mimicking biological ion channels and developing nanosized pH sensing devices.

这项研究表明，通过调节3-氨基丙基二甲基乙氧基硅烷（APTES）和（3-三乙氧基甲硅烷基丙基）琥珀酸酐（TESP-SA）的比例，可以定制玻璃纳米移液器中基于离子电流整流（ICR）的pH响应。用于硅烷化。通过研究纳米移液器的精馏比来优化硅烷化时间和浓度。对于每个APTES / TESP-SA硅烷比率，方差分析（ANOVA）测试表明，纳米移液器的精馏比率在3-8的pH范围内具有良好的线性。此外，用相同浓度的氨基和羧基硅烷化的纳米移液器具有最高的pH响应，为1.06×10 ^-4  pH  ^-1。这种用于定制纳米移液器中基于ICR的pH响应的方法具有潜力和多功能性，可用于模拟生物离子通道和开发纳米pH传感设备等应用中。