AbstractA hybrid material was prepared from graphene oxide and melamine sponge, and modified with phenylboronic acid to obtain a sorbent for the enrichment of the nucleosides cytidine, uridine, inosine, guanosine and adenosine. The loading capacity typically is around 27.8 mg g−1 which is comparable to other sorbents, and highly selectivity for cis-diols is observed even if the concentration of potential interferents is 1500-fold higher. The sorbent was placed in an injector, and the process was operated semiautomatically by using a peristaltic pump. The sorbent is stable and can be re-used six times without decrease in efficiency. It was applied to the selective extraction of the cis-diols (cytidine, uridine, inosine, guanosine, adenosine) from HepG2 cells. It presents good linear between 3 to 5000 μg L−1 and the limits of detection (in HPLC analysis with UV detection) are 1–4 μg L−1. Good recoveries of 85–101% were obtained with spiked HepG2 cells samples, with relative standard deviation of ≤9.9%. Graphical abstractSchematic for the preparation of boronic acid modified graphene oxide/melamine sponge composite for in-syringe solid-phase extraction of nucleosides.

中文翻译：

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使用由氧化石墨烯和三聚氰胺海绵组成的苯基硼酸改性杂化材料选择性、快速和半自动富集核苷

摘要 以氧化石墨烯和三聚氰胺海绵为原料制备杂化材料，并用苯基硼酸修饰得到一种吸附剂，用于富集核苷胞苷、尿苷、肌苷、鸟苷和腺苷。负载能力通常约为 27.8 mg g-1，与其他吸附剂相当，即使潜在干扰物的浓度高 1500 倍，也观察到对顺式二醇的高度选择性。将吸附剂置于注射器中，该过程通过使用蠕动泵半自动操作。吸附剂稳定，可重复使用六次而不会降低效率。应用于HepG2细胞中顺式二醇（胞苷、尿苷、肌苷、鸟苷、腺苷）的选择性提取。它在 3 到 5000 μg L-1 之间呈现出良好的线性，检测限（在带 UV 检测的 HPLC 分析中）为 1-4 μg L-1。加标 HepG2 细胞样品获得了 85–101% 的良好回收率，相对标准偏差≤9.9%。图解摘要用于制备用于注射器内固相提取核苷的硼酸改性氧化石墨烯/三聚氰胺海绵复合材料的示意图。