Objective

Biomaterials for periodontal regeneration may have insufficient mechanical and antimicrobial properties or are difficult to apply under clinical conditions. The aim of the present study was to develop a polymeric bone grafting material of suitable physical appearance and antimicrobial photodynamic activity.

Methods

Two light curable biomaterials based on urethane dimethacrylate (BioM1) and a tri-armed oligoester-urethane methacrylate (BioM2) that additionally contained a mixture of β-tricalcium phosphate microparticles and 20 wt% photosensitizer mTHPC (PS) were fabricated and analyzed by their compressive strength, flexural strength and modulus of elasticity. Cytotoxicity was observed by incubating eluates and in direct-contact to MC3T3-E1 cells. Antimicrobial activity was ascertained on Porphyromonas gingivalis and Enterococcus faecalis upon illumination with laser light (652 nm, 1 × 100 J/cm², 2 × 100 J/cm²).

Results

The compressive strength, flexural strength and elastic modulus were, respectively, 311.73 MPa, 22.81 MPa and 318.85 MPa for BioM1 + PS and 742.37 MPa, 7.58 MPa and 406.23 MPa for BioM2 + PS. Both materials did not show any cytotoxic behavior. Single laser-illumination (652 nm) caused total suppression of P. gingivalis (BioM2 + PS), while repeated irradiation reduced E. faecalis by 3.7 (BioM1 + PS) and 3.1 (BioM2 + PS) log-counts.

Significance

Both materials show excellent mechanical and cytocompatible properties. In addition, irradiation with 652 nm induced significant bacterial suppression. The manufactured biomaterials might enable a more efficient cure of periodontal bone lesions. Due to the mechanical properties functional stability might be increased. Further, the materials are antimicrobial upon illumination with light that enables a trans-mucosal eradication of residual pathogens.

中文翻译：

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牙周再生用抗菌光动力活性生物材料

客观的

用于牙周再生的生物材料可能没有足够的机械和抗菌性能，或者在临床条件下难以应用。本研究的目的是开发一种具有适当物理外观和抗菌光动力活性的聚合物骨移植材料。

方法

制备了两种基于氨基甲酸酯二甲基丙烯酸酯（BioM1）和三臂低聚酯-氨基甲酸酯甲基丙烯酸酯（BioM2）的光固化生物材料，该材料还包含β-磷酸三钙微粒和20 wt％的光敏剂mTHPC（PS）的混合物，并对其压缩性进行了分析。强度，弯曲强度和弹性模量。通过孵育洗脱液并直接接触MC3T3-E1细胞观察到细胞毒性。抗微生物活性确定上牙龈卟啉和粪肠球菌在用激光（652纳米，1×100焦耳/平方厘米照度²，2×100J /厘米²）。

结果

BioM1 + PS的抗压强度，弯曲强度和弹性模量分别为311.73 MPa，22.81 MPa和318.85 MPa，BioM2 + PS的抗压强度分别为742.37 MPa，7.58 MPa和406.23 MPa。两种材料均未显示任何细胞毒性行为。单一激光照射（652 nm）导致牙龈卟啉单胞菌（BioM2 + PS）的总抑制，而重复照射使粪肠球菌减少3.7（BioM1 + PS）和3.1（BioM2 + PS）对数。

意义

两种材料均显示出优异的机械和细胞相容性。另外，用652nm的辐射诱导显着的细菌抑制。制造的生物材料可能能够更有效地治愈牙周骨病变。由于机械性能，功能稳定性可能会增加。此外，该材料在用光照射时具有抗菌性，从而能够经粘膜清除残留的病原体。