To help remedy damage from harmful algal blooms, an attempt was made to isolate an algicidal substance previously observed to be present in mackerel muscle hydrolysate. Crude extract was obtained by cold acetone precipitation, and it dissolved best in water. Through molecular weight cut-off determination and tricine-SDS PAGE, the algicidal substance was determined to be a peptide of <1 kDa. Based on this result, purification was first performed using size exclusion chromatography and preparative reverse phase high-performance liquid chromatography. Then, the active algicidal fraction was applied to an ultra-performance liquid chromatography-electrospray ionization-mass spectrometry system, followed by MS/MS analysis. The algicidal peptide had linear structure consisting of amino acids with sequence NH-KMNF-COOH. Its calculated properties were: molecular weight 538.66 g/mol; isoelectric point 9.91; net charge +1 at pH 7.0; and 50% hydrophobicity. Algicidal ability of the identified peptide was confirmed using synthesized peptide. The LC₅₀ values toward four harmful algal blooming species were 0.69, 0.83, 0.85 and 1.24 mg/ml for Alexandrium fundyense, A. catenella, Heterocapsa triquetra, and Prorocentrum minimum, respectively. There was no coincidence in the sequence of the identified peptide with those of known metabolites in the APD, Norine, CAMP, UniProt and METLIN databases. Consequently, this algicidal substance originating from mackerel protein was deduced to be a novel peptide that can usefully be applied to relieve harmful algal blooms.

为了帮助补救由于有害藻华而造成的损害，人们试图分离出先前观察到的鲭鱼肌肉水解产物中存在的一种藻类物质。粗提取物通过冷丙酮沉淀获得，并且其在水中的溶解效果最佳。通过分子量截断测定和tricine-SDS PAGE，该杀藻类物质被确定为＜1kDa的肽。基于该结果，首先使用尺寸排阻色谱法和制备型反相高效液相色谱法进行纯化。然后，将活性杀藻成分应用于超高效液相色谱-电喷雾电离-质谱系统，然后进行MS / MS分析。杀藻肽具有由序列为NH-KMNF-COOH的氨基酸组成的线性结构。其计算的属性为：分子量538.66 g / mol; 等电点9.91; pH 7.0时净电荷+1; 和50％的疏水性。使用合成的肽确认了鉴定出的肽的杀藻能力。LC对于四种有害藻类开花物种，_50个值分别为：亚历山大藻，链球菌，杂种黑麦草和Prorocentrum最小值，分别为0.69、0.83、0.85和1.24 mg / ml 。在APD，Norine，CAMP，UniProt和METLIN数据库中，鉴定出的肽的序列与已知代谢物的序列没有重合。因此，这种源自鲭鱼蛋白的杀藻物质被推论为一种新型肽，可有效地用于缓解有害的藻华。