In Vivo Incorporation of Azide Groups into DNA by Using Membrane‐Permeable Nucleotide Triesters,ChemBioChem

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In Vivo Incorporation of Azide Groups into DNA by Using Membrane‐Permeable Nucleotide Triesters
ChemBioChem ( IF 2.6 ) Pub Date : 2018-08-08 , DOI: 10.1002/cbic.201800351
Masayuki Tera _{1,

2} , Stella M. K. Glasauer ₁ , Nathan W. Luedtke ₁

Affiliation

Soak, click, and image: “POM‐AmdU” is a new membrane‐permeable, nontoxic, nucleotide monophosphate derivative that is metabolically incorporated into zebrafish DNA by simply adding the compound to their water. The resulting azide‐modified DNA has little or no impact on zebrafish development, and it could be detected by microscopy after azide–alkyne click cycloaddition with a fluorescent alkyne.

中文翻译：

通过使用膜可渗透的核苷酸三酯体内将叠氮化物基团掺入DNA

浸泡，点击和显示图像：“ POM‐AmdU”是一种新型的膜可渗透的无毒核苷酸一磷酸衍生物，只需将其添加到斑马鱼DNA中，即可代谢整合到斑马鱼DNA中。生成的叠氮化物修饰的DNA对斑马鱼的发育几乎没有影响，甚至没有影响，在叠氮化物-炔烃与荧光炔烃单击环加成后，可以通过显微镜检测到。

更新日期：2018-08-08

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