Super‐resolution Imaging of Amyloid Structures over Extended Times by Using Transient Binding of Single Thioflavin T Molecules,ChemBioChem

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Super‐resolution Imaging of Amyloid Structures over Extended Times by Using Transient Binding of Single Thioflavin T Molecules
ChemBioChem ( IF 2.6 ) Pub Date : 2018-08-08 , DOI: 10.1002/cbic.201800352
Kevin Spehar ₁ , Tianben Ding ₂ , Yuanzi Sun _{1,

3} , Niraja Kedia ₁ , Jin Lu ₂ , George R Nahass ₁ , Matthew D Lew ₂ , Jan Bieschke _{1,

4}

Affiliation

Blink, and you′ll see it: Transient amyloid binding imaging resolves amyloid structures at a nanometer scale by using the standard probe, thioflavin T (ThT), without the need for covalent modification or immunostaining of amyloids. Binding dynamics and the blinking of ThT molecules on amyloids enables continuous imaging over extended times without image degradation due to photobleaching, and gives robust imaging for various amyloid structures and imaging conditions.

中文翻译：

利用单个硫黄素 T 分子的瞬时结合对淀粉样蛋白结构进行长时间超分辨率成像

眨眼间，您就会看到：瞬时淀粉样蛋白结合成像通过使用标准探针硫黄素 T (ThT) 解析纳米级的淀粉样蛋白结构，无需对淀粉样蛋白进行共价修饰或免疫染色。 ThT 分子在淀粉样蛋白上的结合动力学和闪烁使得能够长时间连续成像，而不会因光漂白而导致图像退化，并为各种淀粉样蛋白结构和成像条件提供稳定的成像。

更新日期：2018-08-08

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